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Apc conjugated anti human mouse cd49f itga6 antibody

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The APC-conjugated anti-human/mouse CD49f/ITGA6 antibody is a flow cytometry reagent that binds to the CD49f (ITGA6) cell surface antigen. CD49f is a subunit of the integrin α6β1 and is expressed on various cell types, including stem cells, epithelial cells, and some hematopoietic cells. This antibody can be used to identify and characterize CD49f-expressing cells.

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Lab products found in correlation

Accutase, by BD (2 mentions) Lyoplate mouse cell surface marker screening panel, by BD (2 mentions)

Close spelling variants of this product

APC anti-human/mouse CD49f Anti-CD49f-APC CD49f-APC

2 protocols using apc conjugated anti human mouse cd49f itga6 antibody

1

Flow Cytometry Cell Profiling of E0771 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell cultures destined for flow cytometry were dissociated with Accutase (BD Biosciences, USA) to limit cleavage of extracellular receptors. Cells were labeled with the APC-conjugated anti-human/mouse CD49f/ITGA6 antibody (BioLegend, Bethesda, MD, USA, product: 313615) or the APC-conjugated Rat IgG2a,k isotype control (BioLegend, Bethesda, MD, USA, product: 400511) according to manufacturer’s recommendations. The flow cytometry cell surface marker screen was conducted on E0771 cells using the BD Lyoplate Mouse Cell Surface Marker Screening Panel (BD, Franklin Lakes, NJ, USA). The geometric mean of fluorescent values <4 was chosen as the cutoff in accordance with assay negative controls. Values for the entire dataset are contained in Supplementary Table 3.
Berens E.B., Sharif G.M., Schmidt M.O., Yan G., Shuptrine C.W., Weiner L.M., Glasgow E., Riegel A.T, & Wellstein A. (2016). Keratin-associated protein 5-5 controls cytoskeletal function and cancer cell vascular invasion. Oncogene, 36(5), 593-605.
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2

Flow Cytometry Cell Profiling of E0771 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
All cell cultures destined for flow cytometry were dissociated with Accutase (BD Biosciences, USA) to limit cleavage of extracellular receptors. Cells were labeled with the APC-conjugated anti-human/mouse CD49f/ITGA6 antibody (BioLegend, Bethesda, MD, USA, product: 313615) or the APC-conjugated Rat IgG2a,k isotype control (BioLegend, Bethesda, MD, USA, product: 400511) according to manufacturer’s recommendations. The flow cytometry cell surface marker screen was conducted on E0771 cells using the BD Lyoplate Mouse Cell Surface Marker Screening Panel (BD, Franklin Lakes, NJ, USA). The geometric mean of fluorescent values <4 was chosen as the cutoff in accordance with assay negative controls. Values for the entire dataset are contained in Supplementary Table 3.
Berens E.B., Sharif G.M., Schmidt M.O., Yan G., Shuptrine C.W., Weiner L.M., Glasgow E., Riegel A.T, & Wellstein A. (2016). Keratin-associated protein 5-5 controls cytoskeletal function and cancer cell vascular invasion. Oncogene, 36(5), 593-605.
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