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2 protocols using potassium persulphate

1

Bacterial Cellulose-Based Hydrogel Composites

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The monomer, acrylic acid (AA, Sigma-Aldrich, 99%) was distilled at vacuum before use, and the crosslinker, N,N′-methylene-bis-acrylamide (MBA, Sigma-Aldrich, 99%) was used as received. Ammonia aqueous solutions 25% was purchased from Chimopar SA and the radical polymerisation redox system, composed of potassium persulphate (KPS, 99%) and sodium metabisulphide (MS, 97%) was procured from Acros Organics. Bacterial cellulose (BC) was obtained by collaborators from the University “Politehnica” of Bucharest in static culture using fructose as carbon source and pollen as nitrogen source43 and delivered in acetic acid. The foliar chemical fertilizer (with microelements) encapsulated into the new BC-IPN composite hydrogels was KRISTALON and provided in solid state (SF).
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2

Antioxidant, Cytotoxicity, and Antimicrobial Activities

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Glacial acetic acid, methanol, and sodium acetate were purchased from QReC, Selangor, Malaysia. DPPH, 2,4,6-tris(2-pyridyl)-1,3,5-triazine (TPTZ), ABTS reagent, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent, iron(II) sulphate (FeSO4.7H2O), and BHA were obtained from Sigma-Aldrich, Missouri, USA. Mueller Hinton Agar (MHA), Mueller Hinton Broth (MHB), Potato Dextrose Broth (PDB), Sabouraud Dextrose Agar (SDA), and commercial silver nanoparticles (C-AgNP) were acquired from Merck, Darmstadt, Germany. Gentamycin sulphate, nystatin, and hydrochloric acid (HCl) were provided by Biobasic, Ontario, Canada. Glycerol was obtained from System®, Selangor, Malaysia. Potassium persulphate was purchased from Acros Organics, New Jersey, USA. 0.25% Trypsin-EDTA, RPMI 1640, penicillin-streptomycin, foetal bovine serum (FBS), L-glutamine, and Dulbecco's Modified Eagle Medium (DMEM) were acquired from Gibco®, New York, USA. 5-fluorouracil (5-FU) was purchased from Tokyo Chemical Industry, Tokyo, Japan. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) kit was provided by Promega, WI, USA. Phosphate buffer saline (PBS) was obtained from Invitrogen, New York, USA.
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