The crimp contributes significantly to the adaptation of the ligament to biomechanically acting forces18 and can be detected very well in polarization (Fig. 2C ). The quantitative evaluation of the crimp length (μm) was performed manually by two examiners (HM and KH) and double‐checked at a different time point. Interrater reliability was 0.87. Ten individual measurements of crimp length per ROI were performed on polarized microscopic images (Olympus Simple Polarizing attachment BX‐Pol, Tokyo, Japan; Giemsa staining). Counting was performed with the Adobe Photoshop counting tool (Adobe Photoshop CS 4 Extended, Version 11.0, Adobe Systems, San Jose, CA, USA). Length measurement was performed with the Stream Motion 1.9 Software by Olympus. Mean values and standard deviations were determined.
Stream motion 1
Manufactured by Olympus
Sourced in Germany
Stream Motion 1.9 software is a data management and analysis tool developed by Olympus. The software is designed to capture, process, and analyze motion data from various imaging and microscopy applications.
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Lab products found in correlation
4 protocols using stream motion 1
1
Quantifying Ligament Crimp Morphology
2
Transverse Wood Surface Imaging
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The wood increment core of S. rubra Sr2 individual was cut by an electric saw into small wood blocks (~0.7 cm × 0.7 cm × 0.7 cm) corresponding to sapwood, transition zone and heartwood, respectively. Each block was then trimmed with a razor blade to generate a transverse cutting surface of approximately 1 mm × 2.5 mm which was left to be cut with an ultramicrotome (EM UC6, Leica Microsystèmes, SAS, Nanterre, France) using a diamond knife (DIATOME Cryotrim 45°, Leica Microsystèmes, SAS, Nanterre, France). A high cutting speed of 50 mm/s was used considering the dense transverse wood surface and the cutting feed was set at 200 nm. The clearance angle was kept constant at 6° during the sectioning. Optical images of the wood surfaces were acquired at 10×magnification with an Olympus BX51 microscope (Rungis, France) equipped with a motorized scanning stage (Marzhauser Wetzlar GmbH, Wetzlar, Germany) and a SC30 color camera, and monitored by the Olympus Stream Motion 1.9 software. Extended focal imaging (EFI) scanning mode was used to reveal the topography of the wood surface.
3
Wood Surface Characterization Protocol
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Before analysis, the bark was removed and the remaining stem was trimmed with a razor blade to generate a small block of about 1 cm × 1 cm × 1 cm. A transverse surface of approximately 1 mm × 3 mm was obtained with ultramicrotome (EM UC6, Leica Microsystèmes, SAS, Nanterre, France) using a diamond knife (DIATOME Cryotrim 45°, Leica Microsystèmes, SAS, Nanterre, France). The cutting was performed at a speed of 2 mm/s with a fixed cutting feed of 200 nm. The clearance angle was kept constant at 6° during the sectioning. Optical images of wood surfaces were acquired at ×10 magnification with an Olympus BX51 microscope (Rungis, France) equipped with a motorized scanning stage (Marzhauser Wetzlar GmbH, Wetzlar, Germany), a SC30 color camera, and monitored by the Olympus Stream Motion 1.9 software. Extended focal imaging (EFI) scanning mode was applied to improve the image quality.
4
Microbial Antagonism Visualization Protocol
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The competition was performed on a MALDI plate (MTP 384 plate polished steel BC, Bruker Daltonique, Wissembourg, France) by applying a thin layer of MEA (5 mL). Once the agar solution cooled, 2 µL of bacterial suspension (10 4 cells/mL) and 5 mL of mycelial suspension (50 mm 3 mycelium pieces vortexed and sonicated in 1 mL of sterile water) were deposited at 1.5 or 2.5 cm apart from each other. For TOF-SIMS experiments, a round Petri dish (9 cm²) was filled with 15 mL of MEA medium and once cooled, sterilized filter paper (Qualitative, Number 2, Cat.
No 1002125 Whatman TM ) was gently deposited on the medium. A volume of 10 µL of the previous microbial suspension was deposited at a distance of 1.5 or 2.5 cm. Competitions on silicon wafer and ITO coated glass slides were also assessed. All the cultures were incubated at 24 °C for 26 days under ambient light. Optical images of the samples were recorded with an Olympus BX51 microscope (Rungis, France), equipped with 1.25 × to 50 × lenses and a SC30 camera, monitored by Stream Motion 1.9 software (Olympus, Rungis, France).
No 1002125 Whatman TM ) was gently deposited on the medium. A volume of 10 µL of the previous microbial suspension was deposited at a distance of 1.5 or 2.5 cm. Competitions on silicon wafer and ITO coated glass slides were also assessed. All the cultures were incubated at 24 °C for 26 days under ambient light. Optical images of the samples were recorded with an Olympus BX51 microscope (Rungis, France), equipped with 1.25 × to 50 × lenses and a SC30 camera, monitored by Stream Motion 1.9 software (Olympus, Rungis, France).
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