Human tracheal mucosal biopsies were obtained in the operating theater and immediately fixed with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde and placed in 4°C overnight. The samples were then prepared as previsouly described27 (link). Briefly, samples were blocked with 0.1% coldwater fish skin gelatin in 50 mM sodium cacodylate buffer and stained with rabbit polyclonal anti-Mtb antibodies (LS-C72966, LSBio, Inc.), followed by goat anti-rabbit IgG conjugated to 20 nm gold particles (Electron Microscopy Sciences). Samples were washed three times with phosphate buffered saline containing 0.1% Tween 20 (PBS-T) and analyzed with an FEI T-12 transmission electron microscope equipped with a side-mounted digital camera. A total of 30-35 individual cells in each group were imaged to analyze subcellular architecture and presence of bacteria.
Ls c72966
Manufactured by LSBio
LS-C72966 is a laboratory equipment product. It is a precision instrument designed for conducting various scientific experiments and analyses. The core function of LS-C72966 is to perform specific tasks within a controlled laboratory environment.
Automatically generated - may contain errors
2 protocols using ls c72966
1
Imaging Mycobacterial Infections in Human Tracheal Tissue
2
Ultrastructural Detection of Mycobacterium Tuberculosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human tracheal mucosal biopsies were obtained in the operating room and immediately fixed with chilled buffer (50 mM sodium cacodylate [pH 7.4]) containing 2.5% glutaraldehyde and 2.0% paraformaldehyde and placed in 4°C overnight. The samples were then prepared as previously described.27 Briefly, samples were blocked with 0.1% coldwater fish skin gelatin in 50 mM sodium cacodylate buffer and stained with rabbit polyclonal anti‐Mycobacterium tuberculosis (Mtb) antibodies (LS‐C72966, LSBio, Inc., Seattle, WA), followed by goat anti‐rabbit IgG conjugated to 20 nm gold particles (Electron Microscopy Sciences, Hatfield, PA). Samples were washed three times with phosphate buffered saline containing 0.1% Tween 20 and analyzed with an FEI T‐12 transmission electron microscope (FEI, Hillsboro, Oregon) equipped with a side‐mounted digital camera. A total of 30 to 35 individual cells in each group were imaged to analyze subcellular architecture and presence of bacteria.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!