Cyp3a7

Human CYP3A4 (Product Number: 456202), CYP3A7 (Product Number: 456237), Nicotinamide Adenine Dinucleotide Phosphate (NADPH) Regenerating System, and low profile Axygen^® single well reagent reservoir with 384-bottom troughs were purchased from Corning Inc. (Corning, NY). The compound library consists of ~2800 drugs from the National Center for Advancing Translational Sciences (NCATS, a component of the U.S. National Institutes of Health (NIH)) Pharmaceutical Collection ¹⁶ , and ~2200 compounds with drug-like properties selected from the NCATS annotated bioactive collections.

POD was purchased from the Chinese National Institutes for Food and Drug Control (No.111645-200602, Beijing, China) with a purity of > 99.0% as detected with HPLC. NADPH, UDPGA, alamethicin, and GSH were obtained from Sigma-Aldrich (St. Louis, MO). Human liver microsomes (HLMs), mouse liver microsomes (MLMs), human recombinant cytochromes P450 (CYP) CYP1A1, CYP1A2, CYP2C8, CYP2C9, CYP2C9*2, CYP2D6, CYP2A6, CYP2B6, CYP3A5, CYP3A4, CYP3A7, CYP2C19, CYP2E1, CYP4A11, CYP4F12, and flavin-containing monooxygenases (FMO) FMO1, FMO3 and FMO5) were all purchased from Corning Life Sciences (Tewksbury, MA, USA). All of the other reagents were top commercial grade.