Tween® 20 at 1mg/mL and used at a final concentration of 200nM.
Wortmannin (LC Laboratories) was dissolved in DMSO at 5mg/mL and used at a final
concentration of 2μM. Cycloheximide (Sigma) was dissolved in
H2O at 10mg/mL and used at 2μg/mL. NVP-BHS345 (Novartis)
was dissolved in DMSO at 10mM and used at a final concentration of 10μM.
Palmitoylcarnitine was dissolved in DMSO at 10mM and used at 10μM, unless
otherwise stated. The FLipTR probe, dissolved in DMSO 20 (link), was used at a final concentration of 2ng/mL. The
Laurdan dye (6-Dodecanoyl-2-dimethylaminonaphthalene) was maintained as a 2.5mM
stock solution in DMF and used at 2.5μM. The FM™4-64 dye
((N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl)
Pyridinium Dibromide, Thermofisher) was dissolved at 10mM in DMSO and used at a
final concentration of 10μM. For ATP depletion, cells were treated for
10min with a combination of 3mM NaN3 and 50mM 2-deoxy-D-glucose 41 .