Log In Sign up
The largest database of trusted experimental protocols

Three well silicone insert

Manufactured by Ibidi
Visit Supplier
Sourced in Germany

The three-well silicone insert is a laboratory equipment designed to provide a contained environment for cell culture and experimentation. It features three individual wells made of silicone material, allowing for the controlled culturing or testing of multiple samples simultaneously.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Silicone inserts

2 protocols using three well silicone insert

1

Wound Healing Assay with miR-34a Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
A three-well silicone insert (Ibidi GmbH) was used to perform wound-like scratches in each well. Detached miR-34a stable cells were removed with phosphate-buffered saline wash and cultured in the serum-free medium for 24 h. Microscope images of migratory cells were captured (Zeiss AG) and the width of the gap in each scratch was measured using ImageJ software v1.44p (National Institutes of Health).
Hwang T.I., Cuiu Y.C., Chen Y.C., Chen P.C., Tsai T.F., Chou K.Y., Ho C.Y., Chen H.E., Chang P.H, & Chang A.C. (2023). Tumor suppressive functions of hsa-miR-34a on cell cycle, migration and protective autophagy in bladder cancer. International Journal of Oncology, 62(5), 66.
+ Open protocol
+ Expand
2

Wound Healing Assay with VSMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
A wound healing assay was conducted using migration dishes containing a three-well silicone insert (Ibidi GmbH, Gräfelfing, Germany). Upon isolation, 1.5 × 105 VSMCs from both genotypes were seeded and cultured until they grew confluent. For generating a migration gap, the three-well insert was removed, and cells in the different reservoirs were monitored every 24 h for 72 h using phase-contrast microscopy attached to a digital Axiocam MRc Rev.3 camera. To quantify the gap closure, pictures were repeatedly taken from exactly the same gap positions.
Längst N., Adler J., Kuret A., Peter A., Ruth P., Boldt K, & Lukowski R. (2022). Cysteine-Rich LIM-Only Protein 4 (CRP4) Promotes Atherogenesis in the ApoE−/− Mouse Model. Cells, 11(8), 1364.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!