Zinc finger e box binding homeobox 1 zeb1

The 20(S)-Rg3 was provided by the Yiyan Biological Technology Company (Shanghai, China). Rg3 was dissolved in dimethyl sulfoxide (DMSO) in a 200mg/ml stock solution and stored at -20°C and diluted with fresh culture medium before use. An equal volume of DMSO (final concentration <0.1%) was added to the controls. The recombinant human TGF-β was purchased from PeproTech (Rocky Hill, NJ, USA). Primary monoclonal antibodies including rabbit anti-human MMP-2, MMP-9, E-cadherin, N-cadherin, Vimentin, Snail, Slug, Twist, Zinc Finger E-Box Binding Homeobox 1 (ZEB1), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The horseradish peroxidase-conjugated secondary antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The ECM gel (E1270) was obtained from Sigma (St. Louis, MO, USA).

Fetal bovine serum, RPMI 1640, DMEM, MEM culture medium and penicillin and streptomycin were purchased from Gibco (Grand Island, NE, USA). Cordycepin, doxorubicin and MTT were purchased from Sigma (Ronkonkoma, NY, USA). Trypsin was purchased from Beijing Solaibao Technology Co., Ltd.. Dimethyl sulfoxide was purchased from Sinopill Chemical Reagents Co., Ltd.. Primary antibodies against GAPDH, E-cadherin, N-cadherin, Zinc finger E-box binding homeobox 1 (Zeb1), Twist1, NFKB, MAPK8, MMP-9, and cleaved caspase 3 were purchased from Cell Signaling Technology.
The Cordycepin CAS number is 73-03-0, and its HPLC is >98%. The doxorubicin CAS number is 25316-40-9, and its purity is 98.0%–102.0%.