Human ige full length protein

Manufactured by Abcam

Sourced in United States

Human IgE full-length protein is a laboratory reagent used for research purposes. It is the full-length version of the human immunoglobulin E (IgE) protein. IgE is an antibody involved in the body's immune response, particularly in allergic reactions. This protein can be used in various research applications to study the structure, function, and interactions of IgE.

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Lab products found in correlation

Interleukin 3 (il 3), by BioLegend (1 mentions) Stem cell factor (scf), by BioLegend (1 mentions) Fitc anti fcεriα, by BioLegend (1 mentions) Anti cd117 apc, by BioLegend (1 mentions) Europium conjugated streptavidin, by PerkinElmer (1 mentions) Legend max elisa kit, by BioLegend (1 mentions) Anti human ige clone g7 18, by BD (1 mentions)

Close spelling variants of this product

Human IgE

2 protocols using human ige full length protein

Mast Cell Differentiation and Activation Assay

Cited 1 time

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After the primary separation of bone marrow cells from femora and tibiae of wild-type mice, 10 ng/ml IL-3 and 10 ng/mL SCF (Biolegend, California, USA) were added into RPMI-1640 cell culture medium to induce bone marrow cell differentiation to mast cells. After 4-6 weeks culture, flow cytometry analysis was used to separate CD117 and FcεRIα-positive mast cells (APC anti-CD117 and FITC anti-FcεRIα, Biolegend, California, USA) [41 (link)]. The purified mast cells were activated using human IgE full-length protein (Abcam, Massachusetts, USA) overnight and then challenged with 200 μg ovalbumin for 1 h [42 (link)]. Real-time PCR was used to detect the mRNA expression levels of P2Y₆, IL-4, IL-5 and IL-13 on mast cells.

Shi J.P., Wang S.Y., Chen L.L., Zhang X.Y., Zhao Y.H., Du B., Jiang W.Z., Qian M, & Ren H. (2016). P2Y6 contributes to ovalbumin-induced allergic asthma by enhancing mast cell function in mice. Oncotarget, 7(38), 60906-60918.

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Quantification of Cytokines and Immunoglobulins

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Cell culture supernatants were assayed for human or murine IFNγ, IL-4, IL-17A/F and murine GM-CSF using LEGEND MAX™ ELISA kits (BioLegend). Human plasma IgE was captured using anti-human IgE (clone G7-18, BD Biosciences). Human IgE full-length protein (Abcam) was the standard and biotinylated anti-human IgE (clone G7-26, BD Biosciences) and Europium-conjugated streptavidin (Perkin Elmer) were used for detection. Murine plasma IgE was captured using anti-mouse IgE (clone R35-72), with purified mouse IgE as the standard and biotinylated anti-mouse IgE (clone R35-118) for detection (BD Biosciences).

Dendrou C.A., Cortes A., Shipman L., Evans H.G., Attfield K.E., Jostins L., Barber T., Kaur G., Kuttikkatte S.B., Leach O.A., Desel C., Faergeman S.L., Cheeseman J., Neville M.J., Sawcer S., Compston A., Johnson A.R., Everett C., Bell J.I., Karpe F., Ultsch M., Eigenbrot C., McVean G, & Fugger L. (2016). Resolving TYK2 locus genotype-to-phenotype differences in autoimmunity. Science translational medicine, 8(363), 363ra149.

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