The largest database of trusted experimental protocols

Anti phospho akt substrate 23c8d2

Manufactured by Cell Signaling Technology
Sourced in Germany

Anti-phospho-AKT-substrate (23C8D2) is an antibody that recognizes proteins phosphorylated by the AKT kinase. It is a research-use-only product for detecting AKT substrates in cells and tissues.

Automatically generated - may contain errors

2 protocols using anti phospho akt substrate 23c8d2

1

Antibody Characterization for MLH1 Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MLH1 (G168-728) and anti-PMS2 (A16-4) were purchased from Pharmingen (BD Biosciences, Heidelberg, Germany), anti-beta Actin (Clone AC-15) was from Sigma-Aldrich (Munich, Germany). Anti-phospho-AKT-substrate (23C8D2), used for the detection of phosphorylation of MLH1 at amino acid position serine 477 and hereinafter referred to as anti-p-MLH1, was obtained from Cell Signaling (New England Biolabs GmbH, Frankfurt, Germany). Anti-MLH1 (N-20), anti-CK2α (D-10), and anti-Lamin β (C-20) were from Santa Cruz (Santa Cruz Biotechnology, Heidelberg, Germany). Anti-MLH1 (ab74541) (Abcam, Cambridge UK) and anti-Adaptin γ (clone 88/Adaptin γ (RUO)) were from BD Biosciences (BD Biosciences, Heidelberg, Germany).
Anti-fluorescence-labeled anti-rabbit IRDye800CW and anti-fluorescence-labeled anti-mouse IRDye680LT were from LI-COR (LI-COR Biosciences GmbH, Bad Homburg, Germany).
+ Open protocol
+ Expand
2

Immunoblotting for Phospho-MLH1 Detection

Check if the same lab product or an alternative is used in the 5 most similar protocols
Anti-MLH1 (G168-728) and anti-PMS2 (A16-4) were purchased from Pharmingen (BD Biosciences, Heidelberg, Germany). Anti-beta Actin (Clone AC-15) was from Sigma-Aldrich (Munich, Germany). Anti-phospho-AKT-substrate (23C8D2), used for the detection of phosphorylation of MLH1 at amino acid position serine 477 and hereinafter referred to as anti-p-MLH1, was obtained from Cell Signaling (New England Biolabs GmbH, Frankfurt, Germany).
Anti-fluorescence-labeled anti-mouse IRDye680LT and anti-fluorescence-labeled anti-rabbit IRDye800CW were from LI-COR (LI-COR Biosciences GmbH, Bad Homburg, Germany).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!