Superscript 3 rnase h rt and primers

Dulbecco’s Modified Eagle’s Medium and Ham F-12 Nutrient Mix (DMEM/F12), Roswell Park Memorial Institute (RPMI) medium, progesterone, E2, penicillin, streptomycin, DNase type I, and bovine serum albumin were purchased from Sigma Chemical Co (Saint Louis, MO, USA). Fetal calf serum (FCS) was purchased from Invitrogen (Carlsbad, CA, USA). The sPIF (purity: 95%, as documented by HPLC and mass spectrometry) was produced by Biosynthesis (Lewisville, TX, USA). Superscript III RNase H-RT and primers were from Invitrogen, and RNase inhibitor was obtained from AMRESCO (Solon, OH, USA). The Nucleospin RNA II kit was obtained from Machery-Nagel (Düren, Germany). Trypsin was provided by Difco Laboratories (Detroit, MI, USA). Matrigel® was obtained from BD Biosciences (Le Pont-de-Claix, France) and collagenase A was from Boehringer (Mannheim, Germany). The selective, irreversible PI3K inhibitor wortmannin was purchased from Sigma Chemical Co. The suppliers of the various antibodies used here are described in the corresponding paragraphs below.