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Anti taz

Manufactured by Proteintech
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Anti-TAZ is a primary antibody that specifically recognizes the TAZ protein. TAZ is a transcriptional co-activator that plays a role in the Hippo signaling pathway. The Anti-TAZ antibody can be used for applications such as Western blotting and immunoprecipitation to detect and study the TAZ protein.

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Lab products found in correlation

Anti yap, by Proteintech (4 mentions) Pvdf membrane, by Merck Group (2 mentions) Ecl reagent, by Beyotime (2 mentions) Anti gapdh, by Merck Group (2 mentions) Anti e cadherin, by Cell Signaling Technology (2 mentions) Anti hpv16 e6 hpv18 e6, by Abcam (2 mentions) Anti vimentin, by Abcam (2 mentions) Chemidoc xrs system, by Bio-Rad (2 mentions) Anti hpv16 e7, by Abcam (2 mentions) Anti ccl2, by Proteintech (2 mentions) β catenin, by Proteintech (1 mentions) Anti p53, by Proteintech (1 mentions)

Spelling variants of this product

Anti-TM (2 citations) Anti-TAZ antibody (2 citations) Anti-TAZ (23306-1-AP, (2 citations)

4 protocols using anti taz

1

Comprehensive Protein Analysis Protocol

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The whole proteins were extracted using cell lysis buffer. Then, SDS-PAGE was used to separate proteins with different sizes. After that, protein was transferred to a PVDF membrane (Millipore) and target protein was immunoblotted with specific primary antibody. Primary antibodies were listed as follows: anti-E-cadherin (1:5000, mouse anti-human, Cell Signaling Technology Inc.), anti-Vimentin (1:2000, mouse anti-human, Abcam), anti-HPV16 E6 + HPV18 E6 (1:1000, mouse anti-human papillomavirus, Abcam), anti-HPV16 E7 (1:1000, mouse anti-human papillomavirus, Abcam), anti-YAP (1:2000, mouse anti-human, Proteintech), anti-TAZ (1:2000, mouse anti-human, Proteintech), anti-CCL2 (1:2000, mouse anti-human, Proteintech), anti-flag-YAP (1:1000, mouse, Abbkine), anti-GAPDH (1:2000, rabbit anti-human, Sigma-Aldrich). After incubating goat anti-rabbit or goat anti-mouse secondary antibody (MultiSciences), immunoblots were visualized using the chemiluminescence (ECL) reagent (Beyotime Biotechnology) and ChemiDoc XRS+ System (Bio-Rad).
Cao M.X., Zhang W.L., Yu X.H., Wu J.S., Qiao X.W., Huang M.C., Wang K., Wu J.B., Tang Y.J., Jiang J., Liang X.H, & Tang Y.L. (2020). Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression. Journal of Experimental & Clinical Cancer Research : CR, 39, 102.
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2

Immunohistochemical Profiling of Cell Lines

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IHC analysis was performed on the cell-block sections from the cultured cells by using the following primary antibodies: anti-AKT1 antibody (dilution 1:100; Proteintech), p53 (1:100, A nity), anti-YAP (1:100, Proteintech), anti-TAZ (1:100, Proteintech), and β-catenin (1:100, Proteintech).
Peng J., Yu J., Liu J., Wei H., Song H., Shi S., Wang S., Guo D., Li Z., He S., Feng X., Li L., Zhao R., Liu Y., Liu Y., Li J., Zhang R., & Wang W. (2020). Absence of miR-378d Promoted The Malignant Phenotype of ESCC Through The AKT-β-Catenin and Hippo-p53 Signaling Pathway.
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3

Immunostaining of p53, YAP, and TAZ

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About 3×10 4 cells were seeded on the cover glass of sixwell plates and cultured for 48 h. After xing with 4% paraformaldehyde in a 37 ℃ water bath for 15 min to maintain cell morphology, cells were permeabilized in PBS + 0.3% Triton X-100 for 15 min, and incubated with anti-p53 (1:100, Proteintech), anti-YAP (1:100; A nity), and anti-TAZ (1:100; Proteintech) at 4 ℃ overnight, followed by anti-rabbit Cy3labeled secondary antibody (1:150; ABclonal). PBS washing was performed required for each step.
Peng J., Yu J., Liu J., Wei H., Song H., Shi S., Wang S., Guo D., Li Z., He S., Feng X., Li L., Zhao R., Liu Y., Liu Y., Li J., Zhang R., & Wang W. (2020). Absence of miR-378d Promoted The Malignant Phenotype of ESCC Through The AKT-β-Catenin and Hippo-p53 Signaling Pathway.
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4

Protein Extraction and Western Blot Analysis

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The whole proteins were extracted using cell lysis buffer. Then, SDS-PAGE was used to separate proteins with different sizes. After that, gel was transferred to a PVDF membrane (Millipore) and target protein was immunoblotted with speci c primary antibody. Primary antibodies were listed as follows: anti-Ecadherin (1:5000, mouse anti-human, Cell Signaling Technology Inc.), anti-Vimentin (1:2000, mouse antihuman, Abcam), anti-HPV16 E6 + HPV18 E6 (1:1000, mouse anti-human papillomavirus, Abcam), anti-HPV16 E7 (1:1000, mouse anti-human papillomavirus, Abcam), anti-YAP (1:2000, mouse anti-human, Proteintech), anti-TAZ (1:2000, mouse anti-human, Proteintech), anti-CCL2 (1:2000, mouse anti-human, Proteintech), anti-ag-YAP (1:1000, mouse, Abbkine), anti-GAPDH (1:2000, rabbit anti-human, Sigma-Aldrich). After incubating goat anti-rabbit or goat anti-mouse secondary antibody (MultiSciences), immunoblots were visualized using the chemiluminescence (ECL) reagent (Beyotime Biotechnology) and ChemiDoc XRS + System (Bio-Rad).
Cao M., Zhang W., Yu X., Wu J., Qiao X., Huang M., Wang K., Wu J., Tang Y., Jiang J., Liang X., & Tang Y. (2020). Interplay between cancer cells and M2 macrophages is necessary for miR-550a-3-5p down-regulation-mediated HPV-positive OSCC progression.
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