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Biotinylated detection abs

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Biotinylated detection Abs are antibodies that have been conjugated with biotin, a small molecule that can bind to streptavidin or avidin. This allows the antibodies to be detected in various immunoassay and detection applications.

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Lab products found in correlation

Verikine hs mouse interferon beta serum elisa kit, by PBL Assay Science (1 mentions) Mouse cytokine array panel a kit, by R&D Systems (1 mentions)

2 protocols using biotinylated detection abs

1

Quantitative Cytokine ELISA Protocol

Cited 1 time
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Concentrations of IL-6, IL-12, and TNFα in culture supernatants or mouse sera were determined by quantitative ELISA using cytokine-specific coating Abs and biotinylated detection Abs (eBioscience) as previously described (10 (link), 22 (link)). Mouse serum levels of IL-10 and IL-1β were analyzed by ELISA using cytokine-specific coating Abs and biotinylated detection Abs (R&D) as previously described (10 (link), 22 (link)). Levels of IFN-β in mouse sera were measured using the VeriKine-HS™ Mouse Interferon Beta Serum ELISA Kit (PBL Assay Science, Piscataway, NJ) following the manufacturer’s protocol.
Lalani A.I., Moore C.R., Luo C., Kreider B.Z., Liu Y., Morse HC I.I.I, & Xie P. (2014). Myeloid cell TRAF3 regulates immune responses and inhibits inflammation and tumor development in mice. Journal of immunology (Baltimore, Md. : 1950), 194(1), 334-348.
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2

Cytokine and Chemokine Profiling After BCG Immunization

Cited 1 time
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Sera were collected from LMC and M-Traf3-/- mice at day 1 or day 2 after the 2nd and 3rd BCG injections. Serum levels of 40 cytokines and chemokines were measured using a Mouse Cytokine Array Panel A kit (R&D, Minneapolis, MN) following the manufacturer’s instructions (27 (link)). Images of the blots were acquired and quantitative analyses of cytokine/chemokine spots were performed using a low-light imaging system (LAS-4000 mini, FUJIFILM Medical Systems USA, Inc., Stamford, CT) (27 (link)). Concentrations of G-CSF, CXCL-13, S100A8/A9, HMGB1, and CCL22 in mouse sera were determined by quantitative ELISA using specific coating Abs and biotinylated detection Abs (R&D or Biomatik) following the manufacturer’s protocols.
Zhu S., Lalani A.I., Jin J., Sant’Angelo D., Covey L.R., Liu K., Young H.A., Ostrand-Rosenberg S, & Xie P. (2023). The adaptor protein TRAF3 is an immune checkpoint that inhibits myeloid-derived suppressor cell expansion. Frontiers in Immunology, 14, 1167924.
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