The animals were deeply anesthetized and transcardially perfused with 4% PFA. When only NPs were detected, cells or tissues were fixed in cold methanol at −20°C for 10 min, since fixation with paraformaldehyde reduced rhodamine‐related NP fluorescence. Immunohistochemistry was performed on 15–30 μm coronal sections with the following primary antibodies: rabbit anti‐IBA1 (1:500; Wako), rabbit anti‐GFAP (1:250; Dako), rabbit anti‐calbindin28 kDa (1:100; Swant), mouse anti‐DARPP32 or rabbit anti‐DARPP32 (1:100, Epitomics; S. Cruz), and mouse anti‐PMCA ATPase (clone 5F10, 1:500; Thermo Scientific). Alexa Fluor 488‐conjugated goat secondary antibodies (1:1,000; Invitrogen) were used for detection. Sections were counterstained with the nuclear dye Hoechst 33258 or 4′,6‐diamidino‐2‐phenylindole (DAPI) (Invitrogen). Confocal images were acquired with a ZEISS LSM 510 or a LEICA SP5 laser scanning confocal microscopes.
Alexa fluor 488 conjugated goat secondary antibodies
Manufactured by Thermo Fisher Scientific
Alexa Fluor 488-conjugated goat secondary antibodies are fluorescently labeled antibodies that bind to the Fc region of primary antibodies. They are designed for use in immunofluorescence and other fluorescence-based applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 510, by Leica (1 mentions)
Hoechst 33258, by Thermo Fisher Scientific (1 mentions)
Rabbit anti gfap, by Agilent Technologies (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions)
Rabbit anti iba1, by Fujifilm (1 mentions)
Lsm 700 laser scanning microscope, by Zeiss (1 mentions)
2 protocols using alexa fluor 488 conjugated goat secondary antibodies
1
Immunohistochemistry protocol for neuronal markers
2
Immunohistochemistry of Liver Tissue
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Liver tissue was fixed with 4% paraformaldehyde in PBS, embedded in paraffin, and sectioned at a thickness of 3 µm. Sections were stained with hematoxylin-eosin or immunostained with antibodies to Ki67 (550609; BD Biosciences). Immune complexes were detected with Alexa Fluor 488–conjugated goat secondary antibodies (Invitrogen; Li et al., 2004 (link); Yokobori et al., 2009 (link)), and confocal images were acquired with an LSM 700 Laser Scanning Microscope (Carl Zeiss).
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