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Cd9 antibody

Manufactured by Cell Signaling Technology
Sourced in United States

The CD9 antibody is a laboratory reagent used to detect the CD9 protein in biological samples. CD9 is a member of the tetraspanin family of cell surface proteins, which play a role in cell-cell interactions and signal transduction. The CD9 antibody can be used in various immunoassays, such as Western blotting and flow cytometry, to identify and quantify the presence of CD9 in cells or tissues.

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2 protocols using cd9 antibody

1

Phosphorylation-Specific Antibody Assay

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TAK1 (phosphor-Ser192) (#orb7051) and NFKB1 (phosphor-Ser933) antibodies were purchased from Biorbyt (Cambridge, UK). The Anti-NF-kappaB p105 (pS932) phospho antibody (#MBS8210747) was purchased from MyBioSource (San Diego, CA). Mouse anti-chicken glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#AM4300), goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated antibody (#A16078), and radioimmunoprecipitation assay (RIPA) lysis and extraction buffers were purchased from Thermo Fisher Scientific (Waltham, MA). Alexa Fluor 488 goat anti-rabbit IgG (H+L) secondary antibody and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (#A-11008; Carlsbad, CA). Anti-rabbit IgG (H+L) HRP-conjugated antibody was purchased from Promega (#W4011; Madison, WI). The CD9 antibody was purchased from Cell Signaling (#13174; Danvers, MA).
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2

Antibody Immunoblotting Protocol

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Primary antibody sources: mCherry antibody (#43590), GFP antibody (#2956), FKBP1A/FKBP12 antibody (#55104), CD9 antibody (#13174), TSG101 antibody (#72312), and Alix antibodies (#18269) were diluted 1:1000 for western blotting, which was purchased from Cell Signaling Technology (Beverly, MA, USA); mTOR (human FRB Domain) antibody (diluted 1:1000 for WB, ALX-215-065) was procured from Enzo Life Science (Farmingdale, NY, USA); HSP70 antibody (diluted 1:1000 for WB, #242707) was obtained from R&D systems (Minneapolis, MN, USA); Beta actin antibody (diluted 1:1000 for WB, ab13772) was purchased from Abcam (Cambridge, MA, USA). Secondary antibody sources: anti-rabbit (#32460) and anti-mouse secondary antibody (#32430) were obtained from Thermo Fisher Scientific (Wilmington, DE, USA).
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