Phenol red and l glutamine

Manufactured by Vitrocell

Sourced in Brazil

Phenol red and L-glutamine are commonly used reagents in cell culture applications. Phenol red is a pH indicator, while L-glutamine is an essential amino acid required for cell growth and maintenance.

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Lab products found in correlation

Inactivated fetal bovine serum, by Vitrocell (2 mentions) Praziquantel, by Merck Group (1 mentions) Glutaraldehyde solution, by Merck Group (1 mentions) Nsaids, by Merck Group (1 mentions) M199 medium, by Vitrocell (1 mentions) Penicillin g streptomycin sulfate, by Vitrocell (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Hepes buffer, by Merck Group (1 mentions)

Spelling variants of this product

L-glutamine (6 citations)

2 protocols using phenol red and l glutamine

Adult Bioassays for Drug Screening

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All NSAIDs were purchased from Sigma-Aldrich (St. Louis, MO, USA), Cayman Chemical (Ann Arbor, MI, USA) and Toronto Research Chemicals (Toronto, Ontario, Canada). Praziquantel was purchased from Merck (São Paulo, SP, Brazil). Stock solutions for use in adult bioassays in vitro were prepared in Dimethyl sulfoxide (DMSO) at a concentration of 10 mM. All drug tested (n = 73) are in Supplementary Table 1.
Roswell Park Memorial Institute (RPMI 1640) culture medium containing phenol red and l-glutamine, M199 medium, inactivated fetal bovine serum (FBS), penicillin G/streptomycin sulfate, and HEPES buffer were obtained from Vitrocell (Campinas, SP, Brazil). DMSO and glutaraldehyde solution were obtained from Sigma-Aldrich.

Lago E.M., Silva M.P., Queiroz T.G., Mazloum S.F., Rodrigues V.C., Carnaúba P.U., Pinto P.L., Rocha J.A., Ferreira L.L., Andricopulo A.D, & de Moraes J. (2019). Phenotypic screening of nonsteroidal anti-inflammatory drugs identified mefenamic acid as a drug for the treatment of schistosomiasis. EBioMedicine, 43, 370-379.

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Preparation and Characterization of Cardiovascular Drug Solutions

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All chemicals were of reagent grade quality or better, obtained from commercial suppliers and used without further purification. Cardiovascular drugs were purchased from Sigma-Aldrich (St. Louis, MO, USA), Cayman Chemical (Ann Arbor, MI, USA), and Toronto Research Chemicals (Toronto, ONT, Canada). Praziquantel was kindly provided by Ecovet Industria Veterinaria Ltda (Sao Paulo, Brazil). Roswell Park Memorial Institute (RPMI 1640) culture medium containing phenol red, and l-glutamine, penicillin G/streptomycin sulfate solution, and inactivated fetal bovine serum were obtained from Vitrocell (Campinas, Brazil). Dimethyl sulfoxide (DMSO), glutaraldehyde solution, and HEPES buffer were purchased from Sigma-Aldrich. In all in vitro experiments, drugs were solubilized in DMSO (0.5% in RMPI v/v), whereas for in vivo studies, drugs were dissolved in ethanol 2% in water (v/v).

Porto R., Mengarda A.C., Cajas R.A., Salvadori M.C., Teixeira F.S., Arcanjo D.D., Siyadatpanah A., Pereira M.D., Wilairatana P, & de Moraes J. (2021). Antiparasitic Properties of Cardiovascular Agents against Human Intravascular Parasite Schistosoma mansoni. Pharmaceuticals, 14(7), 686.

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