Exosome-containing fractions were diluted into 10-nm filtered PBS and examined for concentration of exosome-sized vesicles and particles by NTA using a Particle Metrix Zetaview Twin PMX-220, according to the manufacturer’s instructions.
Zetaview twin pmx 220
Manufactured by Particle Metrix
Sourced in Germany
The Zetaview Twin PMX-220 is a piece of lab equipment designed for particle size and zeta potential analysis. It utilizes light scattering and electrophoretic light scattering techniques to measure the size and surface charge of particles suspended in a liquid medium.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using zetaview twin pmx 220
1
Exosome Concentration Assessment by NTA
2
Nanoparticle Characterization of Extracellular Vesicles
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The particle size distribution and zeta potential of isolated EVs were assessed using a nanoparticle tracking analysis (NTA) and a Zeta View instrument (ZetaView® TWIN PMX-220, Particle Metrix, Ammersee, Germany), respectively, which utilizes the dynamic light scattering (DLS) technique at 25 °C with a 90° scattering angle. Zeta-View Analysis software was used for data processing as previously described (all the EV samples were prepared by diluting 1:1000 with PBS (particle-free)) [60 (link),61 (link)].
NTA was also preformed using a Nanosight LM10-HS instrument (Malvern Instruments, Malvern, UK). It is configured with a blue laser (488 nm) and sCMOS camera. The samples were diluted as 1:1000 in filtered PBS. Three videos of 60 s were captured with a camera shutter speed fixed at 30.00 ms. The camera level was set to 13, and the detection threshold was set to 5. Between each sample reading, the laser chamber was cleaned thoroughly with particle-free milliQ. The collected videos were analyzed using NTA3.4 software to obtain the mode and mean size distributions, as well as the concentration of particles.
NTA was also preformed using a Nanosight LM10-HS instrument (Malvern Instruments, Malvern, UK). It is configured with a blue laser (488 nm) and sCMOS camera. The samples were diluted as 1:1000 in filtered PBS. Three videos of 60 s were captured with a camera shutter speed fixed at 30.00 ms. The camera level was set to 13, and the detection threshold was set to 5. Between each sample reading, the laser chamber was cleaned thoroughly with particle-free milliQ. The collected videos were analyzed using NTA3.4 software to obtain the mode and mean size distributions, as well as the concentration of particles.
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