Thirty micrograms of protein lysates were electrophoresed on a 10% sodium dodecyl sulfate–polyacrylamide gel and transferred onto a polyvinylidene difluoride membrane (Bio-Rad, Hércules, CA, USA). The primary antibodies were goat polyclonal antibody to GSK-3α/β (Cell Signaling Technology, Danvers, MA, USA) and rabbit polyclonal antibody to GAPDH (Santa Cruz, Dallas, TX USA). After incubation with the appropriate secondary antibodies, proteins were immunodetected with infrared fluorescence (Odyssey Imager LI-COR Biosciences, Lincoln, NE, USA). The results are expressed as the optical density of the samples normalized to that of GAPDH. Samples from three mice per group were tested in two independent experiments.
Odyssey imager biosciences
Manufactured by LI COR
Sourced in United States
The Odyssey Imager by LI-COR Biosciences is a near-infrared fluorescence imaging system used for quantitative Western blot and other fluorescence-based assays. The system utilizes dual-channel detection to simultaneously image two different fluorescent signals.
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Lab products found in correlation
2 protocols using odyssey imager biosciences
1
GSK-3α/β Immunoblotting Protocol
2
Quantifying Phospho-protein Levels via SDS-PAGE
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Sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) was performed on 4–15% (w/v) gradient acrylamide gels (TCXTM, BioRad). Proteins were detected with a near-infrared fluorescence imaging system (Odyssey Imager LI-COR Biosciences). Levels of phospho-proteins, normalised to total protein levels, were determined by analysis of fluorescence intensity using Image Studio V3.1 software.
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