Carfentanil and Carfentanil-d5 for analytical standards were acquired from Toronto Research chemicals (Toronto, Ontario, Canada). NorCarfentanil and norfentanyl-d5 were acquired from Chiron AS (Trondheim, Norway). Chromasolv methanol (MeOH) of LC-MS grade was acquired from Honeywell Riedel-de Haën (Seelze, Germany). Carfentanil HCl used for animal experiments was obtained from NIDA Drug Supply Program (Rockville, MD, USA). A stock solution of 1 mg/mL Carfentanil in sterile saline was prepared, and 100 μL aliquots were stored at −20 °C. Ammonium bicarbonate and sodium metabisulfite were acquired from Sigma-Aldrich (St. Louis, MO, USA). Ammonium formate and formic acid (98%) were acquired from VWR International AS (Oslo, Norway). Nitric acid, ethyl acetate, sodium hydroxide and n-heptane were acquired from Merck (Darmstadt, Germany). Type 1 water (18.2 MΩ) purified with a Synthesis A 10 milli-Q system from Millipore (Billerica, MA, USA) was employed.
Synthesis a 10 milli q system
Manufactured by Merck Group
Sourced in United States
The Synthesis A 10 milli-Q system is a water purification system that produces high-purity water. It uses a multi-stage filtration process to remove impurities, resulting in water that meets the stringent standards required for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Chromasolv methanol meoh of lc ms grade, by Honeywell (3 mentions)
Nitric acid, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Ethyl acetate, by Merck Group (2 mentions)
Formic acid, by Avantor (2 mentions)
Ammonium formate, by Avantor (2 mentions)
N heptane, by Merck Group (2 mentions)
Cyclopropylfentanyl hydrochloride, by Cayman Chemical (2 mentions)
Furanylfentanyl d5, by Cayman Chemical (2 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Sodium metabisulfite, by Merck Group (1 mentions)
Carfentanil, by LGC (1 mentions)
3 protocols using synthesis a 10 milli q system
1
Quantification of Carfentanil and Metabolites
2
Cyclopropylfentanyl Synthesis and Analysis
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Cyclopropylfentanyl hydrochloride for animal experiments was acquired from Cayman Chemical (Ann Arbor, MI, USA). A stock solution of 1 mg/mL cyclopropylfentanyl in sterile saline was prepared, and 300 µL aliquots were stored at –20 °C. Cyclopropylfentanyl hydrochloride and furanylfentanyl-d5 for analytical standards were acquired from Cayman Chemical. Cyclopropylnorfentanyl and norfentanyl-d5 were acquired from Chiron AS (Trondheim, Norway). Chromasolv methanol (MeOH) of LC–MS grade was acquired from Honeywell Riedel-de Haën (Seelze, Germany). Ammonium formate and formic acid (98%) were acquired from VWR International AS (Oslo, Norway). Ethyl acetate, n-heptane, nitric acid, and sodium hydroxide were acquired from Merck (Darmstadt, Germany). Disodium tetraborate decahydrate was acquired from Chemi-Teknik AS (Oslo, Norway). Saline was acquired from Hospira (Lake Forest, IL, USA), whereas sodium heparin was acquired from Thomas Scientific (Thomas Scientific, Swedesboro, NJ, USA). Type 1 water (18.2 MΩ) purified with a Synthesis A 10 milli-Q system from Millipore (Billerica, MA, USA) was employed.
3
Analytical Workflow for Fentanyl Analogues
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Cyclopropylfentanyl hydrochloride and furanylfentanyl-d 5 were acquired from Cayman Chemicals (Ann Arbor, MI, USA). Crotonylfentanyl was acquired from Chiron AS (Trondheim, Norway). Ammoniumformate and formic acid (98%) were acquired from VWR International AS (Oslo, Norway). Chromasolv methanol (MeOH) of LC-MS grade was acquired from Honeywell Riedel-de Haën (Seelze, Germany). Pooled HLMs (XTreme 200 Pool) were acquired from XenoTech (Kansas City, KS, USA). Nicotinamide adenine dinucleotide phosphate (NADPH) regeneration solution A and B were acquired from BD Biosciences (San Jose, CA, USA). Type 1 water (18.2 MΩ) purified with a Synthesis A 10 milli-Q system from Millipore (Billerica, MA, USA) was employed.
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