The morphology of the particles and their composition were investigated using a Quanta 3D FEG SEM equipped with a focused gallium ion beam (FIB) (FEI Corp., Eindhoven, The Netherlands), and an EDX detector (Apollo XV SDD, EDAX). Peptide detection was performed in a Specord S600 UV/Vis spectrophotometer (Analytik Jena). Zeta potential measurements were performed with a Zetasizer NANO ZSP (Malvern, Herrenberg, Germany).
Specord s600 uv vis spectrophotometer
Manufactured by Analytik Jena
Sourced in Germany
The Specord S600 is a UV/Vis spectrophotometer manufactured by Analytik Jena. It is designed to measure the absorption of light in the ultraviolet and visible wavelength ranges. The instrument can be used to quantify the concentration of various substances in liquid samples.
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3 protocols using specord s600 uv vis spectrophotometer
1
Particle Characterization via SEM-FIB-EDX
2
Sodium-Stimulated NADH Oxidation in P. bryantii
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The enzyme assays for sodium stimulation of the NADH oxidation by membranes of P. bryantii B14 were performed at 25 °C in 20 mM Tris-HCl (pH 7.5) with increasing concentrations (0 to 500 µM) of NaCl or KCl, 100 µM NADH dipotassium salt, 100 µM ubiquinone-1, and 50 µg of isolated membranes (obtained as described in 2.4). The initial rates were recorded on a SPECORD® S600 UV VIS spectrophotometer (Analytik Jena, Jena, Germany) and NADH oxidation was followed at 340 nm (ε340 = 6.22 mM−1 cm−1). The residual Na+ concentration in each sample was 40 µM. Sodium concentrations were determined with an Agilent Technologies 200 Series AA spectrometer.
3
Optical Characterization of Nanoparticle Synthesis
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The optical measurements were performed with a SPECORD S600 UV-Vis spectrophotometer from Analytik Jena. The absorbance spectra were recorded in 10 second steps for 60 min starting when the temperature of the batch solution reached 85 1C during the heatup period. The spectra covered the 180-1000 nm wavelength region. Reference spectra were measured using Milli-Q water before every measurement. Spectra of the reactants and the PVP concentration series were also recorded to subtract their contribution from spectra of the synthesized suspension. A typical measurement taken at subsequent stages of the synthesis is illustrated in Fig. 2a.
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