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Fitc coupled annexin 5

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FITC-coupled Annexin V is a fluorescently labeled protein that binds to phosphatidylserine, a phospholipid that is exposed on the surface of cells undergoing apoptosis. It can be used to detect and quantify apoptotic cells in various experimental settings.

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Lab products found in correlation

Facscalibur, by BD (1 mentions) Cellquest pro, by BD (1 mentions) Facstar plus flow cytometer, by BD (1 mentions)

Close spelling variants of this product

Annexin V-coupled fluorescein isothiocyanate (FITC) apoptosis detection kit-1 Annexin V-Coupled Fluorescein Isothiocyanate (FITC) Apoptosis Detection Kit Annexin V-FITC Annexin V (Annexin V-FITC) Annexin V

2 protocols using fitc coupled annexin 5

1

Apoptosis Detection by FACS

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FITC-coupled Annexin V (BD Biosciences) was used to detect apoptotic cells by FACS analysis. Propidium iodide was used for DNA staining. FACS analysis was performed with the FACS Calibur and the CellQuest Pro software (BD Biosciences).
Reustle A., Di Marco M., Meyerhoff C., Nelde A., Walz J.S., Winter S., Kandabarau S., Büttner F., Haag M., Backert L., Kowalewski D.J., Rausch S., Hennenlotter J., Stühler V., Scharpf M., Fend F., Stenzl A., Rammensee H.G., Bedke J., Stevanović S., Schwab M, & Schaeffeler E. (2020). Integrative -omics and HLA-ligandomics analysis to identify novel drug targets for ccRCC immunotherapy. Genome Medicine, 12, 32.
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2

Cyclic Stretch-Induced Membrane Changes

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HPDLCs exposed to 20% cyclic stretch strain for 6 h were washed twice with PBS, and phosphatidylserine exposure and loss of membrane integrity was assessed simultaneously by labeling with FITC-coupled annexin V and propidium iodide (PI) (BD Biosciences, San Jose, CA, USA). Labeling was quantified by flow cytometry using a FACStar Plus flow cytometer (BD Pharmingen, Oakville, ON, USA).
Zhao D., Wu Y., Zhuang J., Xu C, & Zhang F. (2016). Activation of NLRP1 and NLRP3 inflammasomes contributed to cyclic stretch-induced pyroptosis and release of IL-1β in human periodontal ligament cells. Oncotarget, 7(42), 68292-68302.
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