Anti human epcam pe

Transiently transfected KatoIII cells with miR-34a-5p mimic or negative control after 48 h, as well as stably transfected cells were collected by centrifugation at 300×g for 5 min. Antibody staining was performed in 100 µl of phosphate buffer saline (PBS) supplemented with 1% bovine serum albumin (BSA) with the following antibodies: anti-human CD44-FITC (eBioscience, cat.no. 11-0441-81), and anti-human EpCAM-PE (eBioscience, cat.no.12-9326-42). Subsequently, the cells were incubated at 4 °C for 40 min. After being stained, the cells were washed in PBS supplemented with 1% BSA and fixed in 1% formaldehyde. Cells were also treated with appropriate isotype control antibodies (eBioscience). Stained cells were analyzed using flow cytometery. The data were analyzed by FlowJo software 7.6.1 (Tree star, Inc., San Carlos, CA, USA).

Cells were stained with anti‐human EpCAM PE (eBiosciences, CA, USA, http://www.ebioscience.com/) antibody for 20 minutes at RT. After washing with PBS, cells were acquired on BD FACS Calibur (BD Biosciences, San Jones, CA) and data was analyzed using Flow Jo software version 7.5.