Human CRC cell lines SW480 (BeNa Culture Collection, Xinyang, China), SW620 (Procell Life Science & Technology Co., Ltd., Wuhan, China), and HT-29 (Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China) were cultured in RPMI 1640 Medium (Livning Biotechnology Co., Ltd., Beijing, China), LoVo cells (Zhong Qiao Xin Zhou Biotechnology Co., Ltd., Shanghai, China) were cultured in DMEM/F12 (Livning Biotechnology Co., Ltd., Beijing, China), and normal human liver cell line QSG-7701 (Beyotime Institute of Biotechnology, Shanghai, China) was cultured in DMEM/high glucose (Livning Biotechnology Co., Ltd., Beijing, China) containing 10% fetal bovine serum (FBS) (Gibco Life Technologies, San Jose, CA, USA). The cells were cultured in a constant temperature incubator at 37 °C, 5% CO2.
Qsg 7701
Manufactured by Beyotime
Sourced in China
The QSG-7701 is a laboratory equipment designed for general use. It serves a core function of providing a controlled environment for scientific experiments and research. The device specifications and technical details are not available in the scope of this request.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Sw480, by BNCC (1 mentions)
Sw620, by Procell (1 mentions)
Ht 29, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Hep3b, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Huh 7, by Shanghai Institute of Biochemistry and Cell Biology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Lenvatinib, by Beyotime (1 mentions)
Hepg2, by National Collection of Authenticated Cell Cultures (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Dulbecco modified eagle medium (dmem), by Procell (1 mentions)
5 protocols using qsg 7701
1
Cell Culture Conditions for CRC and Liver Cell Lines
2
Acquisition of HCC and Adjacent Tissues
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HCC and adjacent normal tissues were acquired from 45 patients, 33 males and 12 females, who had not received therapy prior to surgery during the period from 2018 to 2020 at the affiliated hospital of Nantong University. The study was conducted in accordance with the Declaration of Helsinki (as revised in 2013). Informed consent was obtained from the patients, and this research was approved by the Ethics Committee of Affiliated Hospital of Nantong University (ethical code: 2018-L006). Samples were frozen in liquid nitrogen immediately and stored at –80 °C for future use. Patients provided written informed consent prior to surgery. HCC cell lines (Hep3B, Huh-7, Li-7, and SUN182) were purchased from Shanghai Institute of Cell Biology (Shanghai, China) and a normal liver cell line (QSG-7701) was purchased from Beyotime (Shanghai, China). Cells were cultured at 37 °C under 5% CO2 in RPMI-1640 or DMEM (Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (FBS; Thermo Fisher Scientific), streptomycin (100 µg/mL) and penicillin (100 U/mL).
3
Establishing Lenvatinib-Resistant HCC Cell Lines
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HCC cell lines (MHCC-97H, LM3, HepG2, and Hep3B) and human normal liver cells (QSG-7701) were respectively cultured in dulbecco’s minimum essential medium (DMEM) or Roswell Park Memorial Institute (RPMI)-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 1% streptomycin/penicillin and 10% FBS (Fetal Bovine Serum) (Thermo Fisher Scientific). The cell cultures were grown at 37 ℃ with a continuous supply of 5% carbon dioxide (CO2). The HCC cell lines were provided by the Cell Bank at UCAS (Chinese Academy of Sciences, China). The QSG-7701 was obtained from Beyotime (Shanghai, China). Lenvatinib (Beyotime, SF5346-10 mM) concentration was progressively increased weekly up to 10 µM. After 30 weeks of treatment, HepG2 Lenvatinib-resistant (LR) cell lines (with mixed cell populations, not from a single clone) were obtained.
4
Culturing Human Cancer Cell Lines
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TE-1 (Human esophageal cancer), HepG2 (human hepatocellular carcinoma) and LS513 (Human colorectal cancer) cell lines were purchased from National Collection of Authenticated Cell Cultures. HEEC (Human esophageal epithelium), NCM460 (Human colon epithelium), SGC-7901 (Human gastric cancer) and GES-1(Human gastric epithelium) cell lines were purchased from IMMOCELL. QSG7701 (Human hepatocyte) was purchased from Beyotime. Each cell line is cultured according to the conditions specified in their respective culture instructions.
5
Hepatocyte Hypoxia-Reoxygenation Model
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Normal human hepatocytes QSG-7701 were purchased from Beyotime Biotechnology (Shanghai, China) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Gibco; Thermo Fisher Scientific, United States) supplemented with 10% fetal bovine serum (Biological Industries, Beit Haemek, Israel) and 1% penicillin-streptomycin (Solarbio, Beijing, China) in a humidified incubator (Thermo Fisher Scientific, United States; 37°C, 5% CO2). The H/R model of hepatocytes was established with some modifications to our previous study (Du et al., 2019). Briefly, experimental group hepatocytes were pretreated with various concentrations of KAE for 24 h before H/R procedure, while the control group was treated with the same volume of the KAE vehicle (DMSO; Solarbio, Beijing China) and maintained in the incubator. After that, all hepatocytes were washed twice with warm PBS and replaced with glucose-free and serum-free DMEM (Balanced with 1% O2, 5% CO2, and 94% N2; Procell, Wuhan, China) 1 h before the hypoxia period. Experimental group cells were then cultured under hypoxic conditions (37°C, 1% O2, 5% CO2, and balanced N2) in an InvivO2 400 hypoxic workstation (Baker Ruskinn, United Kingdom) for 6 h. Then, both groups of hepatocytes were replaced with fresh warm DMEM in the incubator (37°C, 5% CO2) for a 4-h reoxygenation period.
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