Western blot was performed according to the manufacturer's specifications. The primary antibodies used were MVH (ab27591, Abcam, USA), OCT4 (ab18976, Abcam, USA), TNF-α (EPR22598-212, Proteintech, USA), IL-2 (26,156–1-AP, Proteintech, USA), IL-10 (Abcam, ab189392), TGF-β(ab179695, Abcam, USA). SIRT1(ab12193, Abcam, USA), SIRT3 (ab189860, Abcam, USA), p21(28,248–1-AP, Proteintech, USA), p53 (10,442–1-AP, Proteintech, USA), GAPDH (ab181602, Abcam, USA). All the HRP secondary antibodies were obtained from Affinity Biosciences. After accordingly antibody incubation and washing with TBST, PVDF membranes were imaged using an EasySee Western blot kit (DW101–01, TransGen, China). Images AI600 and Image J were used to scan and analyze the images.
P21 28 248 1 ap
Manufactured by Proteintech
Sourced in United States
P21(28,248–1-AP) is an antibody product offered by Proteintech. It is a polyclonal antibody that targets the p21 protein, also known as Cyclin-Dependent Kinase Inhibitor 1 or CDKN1A. This antibody is intended for use in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab18976, by Abcam (1 mentions)
Ab27591, by Abcam (1 mentions)
Ab179695, by Abcam (1 mentions)
Ab12193, by Abcam (1 mentions)
Ab189392, by Abcam (1 mentions)
Ab189860, by Abcam (1 mentions)
Ab181602, by Abcam (1 mentions)
Easysee western blot kit, by Transgene (1 mentions)
Polyvinylidene fluoride membrane, by Merck Group (1 mentions)
P53 60283 2 ig, by Proteintech (1 mentions)
Bax 50599 2 ig, by Proteintech (1 mentions)
2 protocols using p21 28 248 1 ap
1
Protein Expression Analysis with Western Blot
2
Western Blot Analysis of Apoptosis Regulators
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The total protein in cell samples was lysed with Radio immunoprecipitation buffer (RIPA) containing protease inhibitor. Protein concentration was determined by Bicinchoninic acid (BCA) quantitative determination method, and the loading amount was 20 μg. Lysates containing equal amount of protein were separated by 15% or 10% SDS-PAGE and transferred to polyvinylidene fluoride membrane (Millipore). 5% of skim milk was used for blocking and then incubated with primary antibody at 4 °C overnight. Primary antibodies against the following protein were used: p53 (60283-2-Ig, Proteintech); Bcl-2 (68103-1-lg, Proteintech); Bax (50599-2-Ig, Proteintech); p21 (28248-1-AP, Proteintech). The membrane was incubated with HRP-labeled secondary antibody at room temperature for 2 h. Blots were exposed and imaged by Tanon imaging system (5200Multi).
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