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Anti α tubulin tu 02

Manufactured by Santa Cruz Biotechnology
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Anti-α-tubulin (TU-02) is a monoclonal antibody that recognizes α-tubulin, a component of the cytoskeleton in eukaryotic cells. It can be used for the detection and localization of α-tubulin in various applications, such as Western blotting, immunohistochemistry, and immunocytochemistry.

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Lab products found in correlation

Calf intestinal alkaline phosphatase, by New England Biolabs (2 mentions) Anti foxp3 abs clone 150d e4, by Thermo Fisher Scientific (2 mentions) Alexa 647 conjugated anti mouse ig, by Thermo Fisher Scientific (2 mentions) Alexa 555 conjugated anti rabbit ig, by Thermo Fisher Scientific (2 mentions) Clone fjk 16s, by Thermo Fisher Scientific (2 mentions) Gitr clone dta 1, by BioLegend (2 mentions) Ctla 4 clone uc10 4b9, by BioLegend (2 mentions) Anti lamin b m 20, by Santa Cruz Biotechnology (2 mentions) Alexa fluor 594 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Anti γ tubulin, by Merck Group (1 mentions) 96 well tissue culture plate, by Corning (1 mentions)

3 protocols using anti α tubulin tu 02

1

Antibody Characterization for Murine T-cell Studies

Cited 1 time
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mAbs specific for mouse CD3 (clone 145-2C11), CD28 (clone 37.51), or CTLA-4 (clone UC10-4B9) were purchased from Biolegend, as were flourophore-conjugated anti-CD4 (Clone GK1.5), -CD8 (53-6.7), -Foxp3 (clone FJK-16s), -CD25 (Clone PC61), -CD44 (Clone IM7) and –GITR (Clone DTA-1) mAbs. Anti-human CD3 mAb (OKT3) was purified in-house. Polyclonal anti-PKC-θ (sc-212), anti-PKC-η (C-15 and sc-215), anti-PAK (N-20 and sc-882), anti-NFATc1 (7A6), anti-lamin B (M-20), and anti-α-tubulin (TU-02) Abs were obtained from Santa Cruz Biotechnology. Anti-p65 (NF-κB), anti-GIT2 and -αPIX Abs were obtained from Cell Signaling Technology. Anti-Foxp3 Abs (clone 150D/E4 for immunoblotting and clone FJK-16s for flow cytometry) were purchased from eBiosciences. Alexa-647-conjugated anti-mouse Ig and Alexa-555-conjugated anti-rabbit Ig were obtained from Molecular Probes. Digitonin was obtained from EMD Chemicals. Calf intestinal alkaline phosphatase was purchased from New England Biolabs. Recombinant CD86-Fc was previously described46 (link).
Kong K.F., Fu G., Zhang Y., Yokosuka T., Casas J., Canonigo-Balancio A.J., Becart S., Kim G., Yates JR I.I.I., Kronenberg M., Saito T., Gascoigne N.R, & Altman A. (2014). Protein Kinase C-η Controls CTLA-4-Mediated Regulatory T Cell Function. Nature immunology, 15(5), 465-472.
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2

Immunofluorescent Staining of Cytoskeletal Proteins

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Cells were grown on 96-well tissue culture plate (Corning, NY) and permeabilized with 0.5% Triton-X100 in PHEM Buffer (80 mM PIPES, 25 mM HEPES, pH 7.2, 10 mM EGTA, 4 mM MgSO4) for 10 min at room temperature, then fixed in PHEM buffer containing 4% paraformaldehyde for 20 min. Cells were blocked with 3% BSA plus 10% FBS in PHEM, and then incubated with primary antibodies: anti-α-tubulin (TU-02) (Santa Cruz) and anti-γ-tubulin (Sigma) in PBS overnight at 4°C. The secondary antibodies Alexa Fluor® 488 goat anti-rabbit IgG (H+L) or Alexa Fluor® 594 goat anti-mouse IgG (H+L) (Invitrogen) were applied respectively for two hours after the plate were washed with TBS thoroughly. 4′, 6-Diamidino-2-phenylindole (DAPI) staining was applied after secondary antibody incubation. Images were captured with a fluorescence microscopy (Olympus IX3).
Fang Y., Kong Y., Xi J., Zhu M., Zhu T., Jiang T., Frett B., Hu W., Li H.Y., Ma M, & Zhang X. (2016). Preclinical activity of MBM-5 in gastrointestinal cancer by inhibiting NEK2 kinase activity. Oncotarget, 7(48), 79327-79341.
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3

Antibody Characterization for Murine T-cell Studies

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
mAbs specific for mouse CD3 (clone 145-2C11), CD28 (clone 37.51), or CTLA-4 (clone UC10-4B9) were purchased from Biolegend, as were flourophore-conjugated anti-CD4 (Clone GK1.5), -CD8 (53-6.7), -Foxp3 (clone FJK-16s), -CD25 (Clone PC61), -CD44 (Clone IM7) and –GITR (Clone DTA-1) mAbs. Anti-human CD3 mAb (OKT3) was purified in-house. Polyclonal anti-PKC-θ (sc-212), anti-PKC-η (C-15 and sc-215), anti-PAK (N-20 and sc-882), anti-NFATc1 (7A6), anti-lamin B (M-20), and anti-α-tubulin (TU-02) Abs were obtained from Santa Cruz Biotechnology. Anti-p65 (NF-κB), anti-GIT2 and -αPIX Abs were obtained from Cell Signaling Technology. Anti-Foxp3 Abs (clone 150D/E4 for immunoblotting and clone FJK-16s for flow cytometry) were purchased from eBiosciences. Alexa-647-conjugated anti-mouse Ig and Alexa-555-conjugated anti-rabbit Ig were obtained from Molecular Probes. Digitonin was obtained from EMD Chemicals. Calf intestinal alkaline phosphatase was purchased from New England Biolabs. Recombinant CD86-Fc was previously described46 (link).
Kong K.F., Fu G., Zhang Y., Yokosuka T., Casas J., Canonigo-Balancio A.J., Becart S., Kim G., Yates JR I.I.I., Kronenberg M., Saito T., Gascoigne N.R, & Altman A. (2014). Protein Kinase C-η Controls CTLA-4-Mediated Regulatory T Cell Function. Nature immunology, 15(5), 465-472.
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