HPLC was performed using an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump and degasser. The flow rate was set to 1.0 mL min−1 and samples were injected using Agilent 1260 autosampler with a 20 μL injection volume. The temperature of the column was set at 37 °C. The HPLC was fitted with a phenomenex Lunar C18 column (150 × 4.6 mm) with 5 m packing (100Ǻ). Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was ‘far UV’) and consisted of mobile phase A: 100% ACN, 0.04% TFA; mobile phase B: 100% water, 0.04% TFA with a gradient of 5 to 95% ACN over 30 min. All data was elaborated using Prism9.
1260 infinity series stack
Manufactured by Agilent Technologies
The 1260 Infinity Series Stack is a modular high-performance liquid chromatography (HPLC) system designed for a wide range of analytical applications. It features a compact, space-saving design and provides reliable, high-quality data acquisition and system control.
Automatically generated - may contain errors
Lab products found in correlation
1260 variable wavelength detector, by Agilent Technologies (3 mentions)
1260 autosampler, by Agilent Technologies (2 mentions)
1260 binary pump and degasser, by Agilent Technologies (2 mentions)
Lunar c18 column, by Phenomenex (1 mentions)
Trace 1300 isq, by Thermo Fisher Scientific (1 mentions)
Laccase from trametes versicolor, by Merck Group (1 mentions)
Agilent 1260 autosampler, by Agilent Technologies (1 mentions)
C18 column, by Agilent Technologies (1 mentions)
Luna c18, by Phenomenex (1 mentions)
4 protocols using 1260 infinity series stack
1
HPLC Analysis of Organic Compounds
2
Laccase-Catalyzed Polymer Synthesis and Characterization
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Laccase from Trametes versicolor (≥0.5 U/mg) was supplied by Sigma-Aldrich and purified by dialysis against water for one day following lyophilization. NIPAM (97%, Aladdin) was recrystallized from hexane before use to remove the inhibitor. Glycidyl acrylate (GA), tris(2-
propanamide (DOPA-Br) were synthesized according to literature procedure and stored in the freezer under a nitrogen atmosphere. 44, [60] [61] [62] The functional dye, rhodamine B 4-(3-(Nhydroxysuccinimidyloxocarbonyl)-propyl)piperazine amide (Rhod B) was synthesized according to previous procedure for the labelling of laccase directly. 63 Copper(I) bromide (CuBr, 98%, Aladdin) was washed sequentially with acetic acid and ethanol and dried under vacuum. Titanium dioxide (anatase 99.8% metals basis, average particle size: 25 nm, Aladdin) was dried in vacuum at 120 °C for 12 hours before use. was acquired from FL3-TCSPC fluorescence spectrometer (Horiba Jobin Yvon). Gas chromatography -mass spectrometry (GC-MS) was performed using a Thermo fisher spectrometer (Trace 1300-ISQ) with heating rate of GC columns increased from 50 ℃ to 200 ℃ in eight minutes.
The HPLC system was an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump, mixer and degasser. Samples were injected using an Agilent 1260 autosampler and detection was achieved using UV and fluorescence detector.
propanamide (DOPA-Br) were synthesized according to literature procedure and stored in the freezer under a nitrogen atmosphere. 44, [60] [61] [62] The functional dye, rhodamine B 4-(3-(Nhydroxysuccinimidyloxocarbonyl)-propyl)piperazine amide (Rhod B) was synthesized according to previous procedure for the labelling of laccase directly. 63 Copper(I) bromide (CuBr, 98%, Aladdin) was washed sequentially with acetic acid and ethanol and dried under vacuum. Titanium dioxide (anatase 99.8% metals basis, average particle size: 25 nm, Aladdin) was dried in vacuum at 120 °C for 12 hours before use. was acquired from FL3-TCSPC fluorescence spectrometer (Horiba Jobin Yvon). Gas chromatography -mass spectrometry (GC-MS) was performed using a Thermo fisher spectrometer (Trace 1300-ISQ) with heating rate of GC columns increased from 50 ℃ to 200 ℃ in eight minutes.
The HPLC system was an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump, mixer and degasser. Samples were injected using an Agilent 1260 autosampler and detection was achieved using UV and fluorescence detector.
3
HPLC Characterization of Organic Compounds
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HPLC was performed using an Agilent 1260 infinity series stack equipped with an Agilent 1260 binary pump and degasser. The flow rate was set to 1.0 mL min -1 and samples were injected using Agilent 1260 autosampler with a 100 μL injection volume. The temperature of the column was set at 37 °C.
The HPLC was fitted with an Agilent C18 column (100 x 4.6 mm) with 5 micron packing (100Ǻ).
Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was 'far UV') and consisted of mobile phase A: 100 % ACN, 0.04 % TFA; mobile phase B: 100 % water, 0.04 % TFA with a gradient of 1 to 80 % ACN over 30 minutes.
The HPLC was fitted with an Agilent C18 column (100 x 4.6 mm) with 5 micron packing (100Ǻ).
Detection was achieved using an Agilent 1260 variable wavelength detector. UV detection was monitored at λ = 309 nm. Methods were edited and run using Agilent OpenLAB online software and data was analysed using Agilent OpenLAB offline software. Mobile phase solvents used were HPLC grade (ACN was 'far UV') and consisted of mobile phase A: 100 % ACN, 0.04 % TFA; mobile phase B: 100 % water, 0.04 % TFA with a gradient of 1 to 80 % ACN over 30 minutes.
4
HPLC and ESI-TOF Mass Spectrometry Analysis
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High performance liquid chromatography (HPLC). Chromatograms were obtained on an Agilent 1260 Infinity series stack equipped with an Agilent 1260 variable wavelength detector. For analytical, the HPLC was fitted with a Phenomenex Luna ® C18 (250 × 4.6 mm) with 5 μm micron packing (100 Å). For semi-preparative purification, the HPLC was fitted with a Phenomenex Luna ® C18 (250 × 4.6 mm) with 5 μm micron packing (100 Å). Mobile phase A consisted of water containing 0.05 % TFA, mobile Phase B consisted of methanol containing 0.05 % TFA. The gradient used for HPLC analysis was increased from 5% to 95 % B in 30 minutes. Detection was achieved via monitoring at 280 nm (Tryptophan absorbance). Purity was calculated using integration of the area under the curve using Agilent OpenLab ® software Mass spectrometry (ESI-TOF). Electrospray ionisation-time of flight mass spectra of samples dissolved in pure methanol were recorded on a Bruker Micro-TOF using positive ionisation mode and a recording range from 500 to 1500 m/z.
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