Spike data were sorted into individual neurons using Offline Sorter x64 V3 (Plexon). For each tetrode recording of spikes, the data was manually sorted into single units using spike features of each of the four channels of the tetrode (e.g. principle component analysis or peak amplitude of each channel). LC call and CA1 pyramidal cell firing rate by state was analyzed using a repeated measures two-way ANOVA with Sidak post hoc.
Offline sorter x64 v3
Manufactured by Plexon
The Offline Sorter x64 V3 is a software tool designed for the analysis and sorting of electrophysiological data. It is capable of processing large datasets and provides users with advanced spike sorting capabilities.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using offline sorter x64 v3
1
Offline Sorting of Tetrode Spikes
2
Dopaminergic Neuron Firing Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single neurons were isolated by offline cluster procedures (Offline Sorter x64 V3; Plexon). Cells with a baseline firing frequency of less than 0.1 Hz over the whole recording were not considered for analysis (identical to Van der Plasse et al.28 (link)). Neurons were classified as DA neurons based on their response to the dopamine 2 receptor agonist apomorphine (⩾20% reduction in firing frequency with respect to pre-treatment activity).30 (link)To exclude effects of the injection procedure on neuronal activity, data from 30 s before and until 30 s after injections were not included in the analyses. Treatment-induced changes in baseline firing frequency were analyzed with a one-way ANOVA and post hoct-test. Burst activity was quantified by computing the fraction of total spikes that occurred within a burst, in accordance with earlier used burst criteria.30 (link), 31 (link)
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!