Dioleoyl-PI(4,5)P2 and neutral phospholipids such as dioleoylphosphatidylcholine (DOPC), dipalmitoyl-phosphatidylcholine (DPPC), and Topfluor Cholesterol (23-(dipyrrometheneboron difluoride)-24-norcholesterol were purchased from Avanti (Alabaster, AL). GloPIPs BODIPY TMR-PI(4,5)P2 C16 was purchased from Echelon Biosciences (Salt Lake City, UT). The concentrations of unlabeled lipid stock solutions were routinely monitored by a phosphorus assay as described elsewhere.27 Tris, EGTA, CaCl2, and KCl were purchased from Fisher Scientific (Hampton, NH). Laurdan was from AnaSpec Inc. (Fremont, CA), and dithiothreitol (DTT) was purchased from Research Product Int. Corp. (Mt. Prospect, IL). Dihydrocholesterol (DChol) was purchased from Sigma-Aldrich (St. Louis, MO) and was used instead of cholesterol to avoid unwanted artifacts resulting from the photo-oxidation of cholesterol.28 –30 (link)
Dihydrocholesterol dchol
Manufactured by Merck Group
Dihydrocholesterol (DChol) is a laboratory reagent used in various biochemical and research applications. It is a naturally occurring sterol molecule that serves as a precursor for the synthesis of other steroids and cholesterol-related compounds. DChol can be utilized in assays, experiments, and analytical procedures where the identification, quantification, or study of sterol-related processes is required.
Automatically generated - may contain errors
Lab products found in correlation
Cacl2, by Thermo Fisher Scientific (1 mentions)
Potassium chloride (kcl), by Thermo Fisher Scientific (1 mentions)
Laurdan, by AnaSpec (1 mentions)
Ethylene glycol tetraacetic acid (egta), by Thermo Fisher Scientific (1 mentions)
Diic18, by Thermo Fisher Scientific (1 mentions)
Milli q system, by Merck Group (1 mentions)
1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions)
Dipalmitoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Dilauroyl phosphatidylcholine, by Avanti Polar Lipids (1 mentions)
2 protocols using dihydrocholesterol dchol
1
Lipid Characterization and Modification Protocol
2
Lipid Monolayer Characterization in Saline
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Dilauroyl phosphatidylcholine (DLPC), dipalmitoyl phosphatidylcholine (DPPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), the lipophilic uorescent probe L,a- phosphatidylethanolamine-N-lissamine rhodamine B sulfonylammonium salt and chicken egg-transphosphatidylated (RhhoPEegg) were purchased from Avanti Polar Lipids. DiIC18 (1,1 0 -dioctadecyl-3,3,3 0 ,3 0 -tetramethylindocarbocyanine perchlorate) was purchased from Molecular Probes and dihydrocholesterol (dchol, a less hydrolyzable cholesterol analog 22 ) was obtained from Sigma. 1-Palmitoyl-2-lauroyl-sn-glycero-3phosphocholine was a generous gi from Dr Bruno Maggio.
Solvents and chemicals were of the highest commercial purity available. The water used for the subphase was from a Milli-Q system (Millipore), 18 MU cm and lipid monolayers were prepared and characterized on subphases of 0.15 M NaCl at 21 AE 1 C.
Solvents and chemicals were of the highest commercial purity available. The water used for the subphase was from a Milli-Q system (Millipore), 18 MU cm and lipid monolayers were prepared and characterized on subphases of 0.15 M NaCl at 21 AE 1 C.
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