After Cuc B incubation, cells and transfected cells were washed with PBS and fixed with 70% ethanol solution overnight at −20°C. After PBS washes the cells were stained with 5 µg/ml RNase and 20 µg/ml PI (propidium iodide/RNase A staining buffer, Invitrogen) in the dark at 37°C for 30 min and then analyzed with a flow cytometry (Becton Dickinson FACS Canto™, Franklin Lakes, NJ). At least 10,000 events were counted for each sample. The DNA content in the G0/G1, S, and G2/M phases were analyzed using ModFit 161 LT version 3.0 software (Verity Software House, Topsham, USA).
Modfit 161 lt version 3
Manufactured by Verity Software House
Sourced in United States
ModFit 161 LT version 3.0 is a software application for cell cycle analysis. It provides tools for DNA content analysis of flow cytometry data.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using modfit 161 lt version 3
1
Cell Cycle Analysis by Flow Cytometry
2
Cell Cycle Analysis by Flow Cytometry
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Cells seeded into 6-well plates were incubated with indicated concentrations of LCA for 24 h. Cells were harvested, washed with PBS, as well as fixed in 70% ethanol and stored at 4 °C overnight. Cells were then stained in PBS containing 5 μg/mL RNase and 20 μg/mL PI in the dark at room temperature for 30 min and analyzed using a flow cytometry (Becton Dickinson FACS Canto, Franklin Lakes, NJ). 10,000 events were recorded for each sample. The DNA content was analyzed using ModFit 161 LT version 3.0 software (Verity Software House, Topsham, USA).
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