Rabbit anti wave3

Rabbit anti-WAVE3 (1:1000) and mouse anti- β-catenin (1:1000) were from Cell Signaling Technologies (Danvers, MA). Goat horseradish peroxidase-conjugated anti-mouse IgG (1:5000) and goat horseradish peroxidase-conjugated anti-rabbit IgG (1:5000) from Calbiochem (San Diego, CA); and Alexa 488-conjugated anti-rabbit IgG and Alexa 568-conjugated anti-mouse IgG are from Invitrogen (Waltham, MA). Vecta-shield with 4′,6-diamidino-2-phenylindole was from Vector Laboratories (Newark, CA).

Rabbit anti-WAVE3 (1∶1000), rabbit anti-MMP9 (1∶1000), rabbit anti-MMP2 (1∶1000), rabbit anti-p38 (1∶1000), rabbit anti phospho p38 (1∶1000), rabbit phospho ERK (1∶1000), rabbit ERK (1∶1000), rabbit anti phospho AKT (Ser473; 1∶1000), rabbit anti panAKT (1∶1000), rabbit anti Phospho JNK (1∶1000), rabbit anti-JNK (1∶1000), mouse anti-phospho p65 (Ser536) (1∶1000), rabbit anti-Cortatcin are from Cell Signaling Technologies. (Danvers, MA); mouse anti-GFP, mouse anti-WAVE2 (1∶3000), mouse anti-WAVE1 (1∶3000) are from Santa Cruz Biotechnology Inc (Santa Cruz, CA); rabbit anti-NFκB p65 (1∶5000) from Biolegend (San Diego, CA), rabbit anti NAP1 (1∶2000), rabbit anti ABI1 (1∶5000), rabbit anti CYFIP2 (1∶3000), mouse anti-actin (1∶5000), rabbit anti-Myc (1∶1000) are from Sigma-Aldrich (St. Louis, MO); goat horseradish peroxidase-conjugated anti-mouse IgG (1∶5000) and goat horseradish peroxidase-conjugated anti-rabbit IgG (1∶5000) from Calbiochem; and Alexa 488-conjugated anti-rabbit IgG and Alexa 568-conjugated anti-mouse IgG, Alexa 568-conjugated phalloidin are from Invitrogen. Vecta-shield with 4′,6-diamidino-2-phenylindole was from Vector Laboratories (Burlingame, CA). Gel electrophoresis reagents were from Bio-Rad (Hercules, CA).