Nucleic acid extraction was performed with the ABI MagMax96 Express automated instrument from one of the 4 aliquots prepared from the original sample (see Figure 1 ). Gene expression of interleukin (IL)-6, IL-10, IL-13, IL-17, IL-25, IL-33, Interferon: alpha; beta; gamma; and lambda and IL-8 was measured using exon-spanning primers and probes according to the manufacturer’s instructions (TaqMan™, Applied Biosystems). All values were normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH) housekeeping gene.
Magmax96 express
Manufactured by Thermo Fisher Scientific
The MagMax96 Express is a magnetic bead-based nucleic acid extraction system designed for high-throughput sample processing. It is capable of extracting DNA, RNA, and proteins from a variety of sample types. The system utilizes automated liquid handling to provide consistent, reliable results with minimal hands-on time.
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Lab products found in correlation
3 protocols using magmax96 express
1
Cytokine Expression Profiling
2
Viral Identification using Automated Nucleic Acid Extraction
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Using an aliquot of Amies transport media plus MagMax lysis/binding buffer, nucleic acid extraction was performed for viral identification using the ABI MagMax96 Express automated instrument and the MagMax 96 Viral Isolation Kit (Thermo Fisher, AMB 18365) (23 (link), 49 ). Adenovirus, influenza subtypes A/B/C, human metapneumovirus (HMPV), human rhinovirus (HRV), parainfluenza virus (PIV) subtypes 1–4, Enterovirus D68, and respiratory syncytial virus (RSV) were tested for using individual real-time qRT-PCR assays. A Ct threshold of 40 was used for all viruses and positive and negative controls were included in each run.
3
Cytokine Expression Profiling
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