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Syndecan 1 primary antibody mouse monoclonal antibody

Manufactured by Santa Cruz Biotechnology

Syndecan-1 primary antibody (mouse monoclonal antibody) is a laboratory reagent used in research applications. It is an antibody that specifically binds to the Syndecan-1 protein, which is a cell surface proteoglycan. This antibody can be used to detect and study the expression of Syndecan-1 in various biological samples.

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2 protocols using syndecan 1 primary antibody mouse monoclonal antibody

1

Quantifying Pulmonary Syndecan-1 Levels

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To detect pulmonary syndecan-1, lungs were sectioned and then incubated with syndecan-1 primary antibody (mouse monoclonal antibody; Santa Cruz Biotechnology) followed by incubation with secondary antibody (goat anti-mouse Alexa Fluor 488; Life Technologies, Eugene, OR). Four random images were taken from each lung section with a fluorescent microscope at 200X and immunofluorescence was quantified and normalized against total alveolar tissue present using Image J software. Results are reported as RFU.
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2

Quantifying Pulmonary Syndecan-1 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
To detect pulmonary syndecan-1, lungs were sectioned and then incubated with syndecan-1 primary antibody (mouse monoclonal antibody; Santa Cruz Biotechnology) followed by incubation with secondary antibody (goat anti-mouse Texas Red; Life Technologies). Random images were taken from each lung section with a fluorescent microscope at 200X and quantified using Image J software. Results are reported as RFU.
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