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Iview dab detection system

Manufactured by Roche
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The IView DAB detection system is a laboratory equipment product designed for the visualization of specific target molecules in biological samples. It utilizes the diaminobenzidine (DAB) chromogen to produce a brown-colored precipitate, enabling the identification and localization of the target analytes.

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Lab products found in correlation

Benchmark xt platform, by Roche (1 mentions) Cell conditioning solution cc1, by Roche (1 mentions)

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DAB detection system (6 citations) IView DAB (4 citations) IView detection system (2 citations)

2 protocols using iview dab detection system

1

EPCAM Immunohistochemical Staining Protocol

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Biopsy specimens were fixed in formalin, routinely processed, and embedded in paraffin. Sections were stained for routine light microscopic evaluation by hematoxylin and eosin (H&E) stain. EPCAM expression was evaluated by using MOC-31 antibody (Cell Marque Corporation) at 1:50 dilution using a Ventana Benchmark XT platform. A mild antigen retrieval was performed using the Ventana proprietary CC1 cell conditioning solution, followed by detection with the Ventana iView DAB detection system and hematoxylin counterstain.
Bodian D.L., Vilboux T., Hourigan S.K., Jenevein C.L., Mani H., Kent K.C., Khromykh A., Solomon B.D, & Hauser N.S. (2017). Genomic analysis of an infant with intractable diarrhea and dilated cardiomyopathy. Cold Spring Harbor Molecular Case Studies, 3(6), a002055.
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2

Evaluation of PTEN Expression

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PTEN immunohistochemistry was performed using the Ventana Benchmark XT autostainer for anti-PTEN (138G6, Cell Signaling Technology, Danvers, MA) with the iVIEW DAB detection system (Ventana Medical Systems, Inc., Tucson, AZ). Absence of staining of tumor cell cytoplasm was classified as loss of expression whereas faint staining was considered as low PTEN expression.
Brana I., Pham N.A., Kim L., Sakashita S., Li M., Ng C., Wang Y., Loparco P., Sierra R., Wang L., Clarke B.A., Neel B.G., Siu L.L, & Tsao M.S. (2017). Novel combinations of PI3K-mTOR inhibitors with dacomitinib or chemotherapy in PTEN-deficient patient-derived tumor xenografts. Oncotarget, 8(49), 84659-84670.
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