Native page novex 4 16 bis tris gels

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Native PAGE Novex 4-16% Bis-Tris Gels are electrophoresis gels designed for the separation and analysis of native, non-denatured proteins. The gels feature a polyacrylamide matrix with a gradient of 4-16% for the effective separation of a wide range of protein sizes.

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Lab products found in correlation

Native mark unstained standard, by Thermo Fisher Scientific (1 mentions) Nativepage sample prep kit, by Thermo Fisher Scientific (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Trans blot turbo transfer system, by Bio-Rad (1 mentions)

Close spelling variants of this product

Novex 4–16% Bis-Tris gels 4–16% Native PAGE Bis-Tris gels Bis-Tris native PAGE Bis-Tris Native gel Novex Bis-Tris gels Bis-Tris PAGE gel Bis-Tris gels 6% native PAGE Novex gels PAGE gels

2 protocols using native page novex 4 16 bis tris gels

Molecular Mass Determination of LBCZ_0230 via Blue Native PAGE

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An analysis of blue native PAGE³³⁾ (link) was performed under nondenaturing conditions to determine the molecular mass of LBCZ_0230. The analytical sample was prepared using a Native PAGE Sample Prep Kit (Life Technologies Corporation, Carlsbad, CA, USA). Electrophoresis was conducted on Native PAGE Novex 4-16 % Bis-Tris Gels (Life Technologies) at 150 V for 115 min on ice. Molecular mass was calibrated using Native Mark Unstained Standard (Life Technologies).

Saburi W., Ota T., Kato K., Tagami T., Yamashita K., Yao M, & Mori H. (2023). Function and Structure of Lacticaseibacillus casei GH35 β-Galactosidase LBCZ_0230 with High Hydrolytic Activity to Lacto-N-biose I and Galacto-N-biose. Journal of Applied Glycoscience, 70(2), 43-52.

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Native PAGE Analysis of Mitochondrial Complexes

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Isolated mitochondria were solubilized in the extraction buffer (1.5 M aminocaproic acid, 50 mM Bis-Tris, 0.5M EDTA and pH 7) containing 1.3% lauryl maltoside or 8 g digitonin / g protein. Samples comprising 20–30 μg of protein were then mixed with the sample buffer (0.75 M aminocaproic acid, 50 mM Bis-Tris, 0.5M EDTA, pH 7, 5% Serva-Blue G-250 and 12% glycerol) and loaded on the precast NativePAGE Novex 4–16% Bis-Tris gels (Life Technologies) and run overnight at a constant voltage of 25 V. Separated protein complexes were then transferred to the PVDF membrane (Millipore), using the Trans-Blot Turbo transfer system (Biorad, Hercules, CA, USA). CII was detected with the anti-SDHA (2E3) antibody (Abcam, AB14715-200).

Kluckova K., Sticha M., Cerny J., Mracek T., Dong L., Drahota Z., Gottlieb E., Neuzil J, & Rohlena J. (2015). Ubiquinone-binding site mutagenesis reveals the role of mitochondrial complex II in cell death initiation. Cell Death & Disease, 6(5), e1749-.

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