Hiscript q select rt supermix for the qpcr kit

According to the manufacturer’s protocol, total RNA was isolated from murine plasma and HT-22 cells using TRIzol (Invitrogen, Carlsbad, CA, USA). The RNA was then reverse transcribed with a stem-loop RT primer (RiboBio, Guangzhou, China) using HiScript Q Select RT SuperMix for the qPCR kit (Vazyme, Piscataway, NJ, USA) and quantified using AceQ qPCR SYBR Green master mix (Vazyme, Piscataway, NJ, USA). The levels of miR-125b-5p mRNA were determined via qRT-PCR and were normalized to the level of Gapdh. For mature miRNAs, the RNA was reverse transcribed with a stem-loop RT primer (RiboBio, USA) using HiScript Q Select RT SuperMix for the qPCR kit (Vazyme, Piscataway, NJ, USA). The cDNA was then set up in a qPCR reaction using AceQ qPCR SYBR Green master mix (Vazyme, Piscataway, NJ, USA). Each procedure was performed in triplicate. The primers used to amplify the circRNAs, and mRNA transcripts, were synthesized by Invitrogen (Carlsbad, CA, USA). The sequences of the primers are listed in Table S1.