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Hrp conjugated goat anti rabbit igg secondary antibodies

Manufactured by Affinity Biosciences
Sourced in China

HRP-conjugated Goat Anti-Rabbit IgG secondary antibodies are used for the detection and quantification of target proteins in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. These antibodies are conjugated with the enzyme Horseradish Peroxidase (HRP), which allows for the amplification and visualization of the signal produced upon binding to the target.

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2 protocols using hrp conjugated goat anti rabbit igg secondary antibodies

1

Lipid-based Nanoparticle Formulation and Evaluation

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Raddeanin A (RA, purity≥98%), trehalose (purity≥98%), Rhodamine B (Rh, purity≥98%), HSPC (purity≥95%), and CHO (purity≥98%) were purchased from Yuanye Bio-Technology (Shanghai, China). DSPE-mPEG2k was purchased from Cayman Chemical Co. (Ann Arbor, MI, USA). The Annexin V-FITC/PI cell apoptosis detection kit and TUNEL Cell Apoptosis Detection Kit were purchased from TransGen Biotech (Beijing, China). The MTT reagent kit, hematoxylin-eosin (H&E) Staining Kit, and Masson’s trichrome staining kit were purchased from Solarbio Science and Technology Company (Beijing, China). Antibodies against GAPDH (Cat# 10494-1-AP) and Bcl-2 (Cat# 26593-1-AP) were purchased from Proteintech (Wuhan, Hubei, China). Anti-cleaved-Caspase-3 antibody (Cat#AF7022), Ki67 (Cat#AF0198), and HRP-conjugated Goat Anti-Rabbit IgG secondary antibodies (Cat#S0001) were purchased from Affinity Bioscience (Changzhou, Jiangsu, China). Anti-HMGB1 (Cat#ab18256) and anti-AR (Cat#ab74272) antibodies were purchased from Abcam (Cambridge, MA, USA). The PSA Elisa Kit (Cat#MM-0469M1) was purchased from Jiangsu Meimian Industrial Co., Ltd. (Jiangsu, China).
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2

Immunohistochemical Analysis of Mouse Tissues

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Mice tissue sections (5 μm) were permeabilized with Triton X-100 in PBS, then blocked with 10% goat serum, and incubated with anti-CX3CL1, anti-GPX4, and anti-PCNA antibodies at 4 °C overnight. Then the sections were incubated with HRP-conjugated goat anti-rabbit IgG secondary antibodies (Cat#S0001, Affinity Biosciences). After counterstaining, sections were dehydrated and imaged using a microscope.
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