The colonies were sub-cultured on Middlebrook 7H10 solid agar (Becton Dickinson, Le Pont de Claix, France). Matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) profile did not match any of the profiles entered in the Bruker database (version December, 2015). Optical microscopy was performed using a Leica DM 2500 microscope (Leica, Wetzlar, Germany) after Gram staining and Ziehl-Neelsen staining and pictures were taken using a Nikon Digital Sight DS-U1 Camera System (Nikon, Tokyo, Japan). For electron microscopy, 10 µL of a bacterial suspension in PBS were deposited on a 400-mesh Formvar carbon-coated copper grid (Euromedex, Strasbourg, France) for 10 minutes after the glow discharge. The sample was contrasted using a 5% ammonium molybdate solution. Images were collected using a Tecnai G2 (FEI, Munich, Germany), operating at 200 keV and bacteria were measured using Image J software, version 1.
Digital sight ds u1 camera system
Manufactured by Nikon
Sourced in Japan
The Nikon Digital Sight DS-U1 Camera System is a digital camera designed for use with microscopes. It captures high-quality images and video to document microscopic samples. The system includes a camera unit, software, and necessary cables for connection to a computer.
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Lab products found in correlation
2 protocols using digital sight ds u1 camera system
1
Bacterial Characterization by Microscopy
2
Microscopic Imaging Techniques
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Microphotographs were taken with an Olympus BX51 microscope (Olympus Corp., Shinjuku, Japan) equipped with a Nikon Digital Sight DS-U1 camera system (Nikon Corp., Shinjuku, Japan), a Zeiss Axioplan 2 Imaging fluorescence microscope (Carl Zeiss AG, Oberkoschen, Germany), and a Leica confocal laser-scanning microscope (Leica TCS SP2; Leica Microsystem GmbH, Wetzlar, Germany).
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