Evo ma10 electron microscope

During the field survey (2008–2012), several edible mushrooms were collected from the forest floor of West Bengal, India accompanied by tribal mushroom hunters of the regions. Among the basket of mushrooms which they usually gather for their regular dishes, a unique mushroom, commonly called “JHAL PATRA” (JHAL = because of its acrid taste; PATRA = Mushroom) was chosen and brought to the laboratory for thorough taxonomic investigation. Detailed microscopic work was performed using the protocol of Buyck & Adamcik (2011) (link) and it was identified as Russula senecis S. Imai using standard literatures (Imai, 1938 ; Zhishu, Guoyang & Taihui, 1993 ; Das, 2009 ). Colour codes and terms (mostly) follow the Royal Botanic Gardens Edinburgh colour chart (Henderson, Orton & Watling, 1969 ). Scanning Electron Microscope (SEM) illustrations of basidiospores were carried out with a Zeiss EVO-MA10 electron microscope (Zeiss, Oberkochen, Germany) at the Centre for Research in Nanoscience and Nanotechnology, University of Calcutta, Kolkata, India. After thorough microscopic work, the specimen voucher had been deposited in Calcutta University Herbarium (CUH).

For the analysis by scanning electron microscopy (SEM), the right tibiae were kept in
absolute ethyl alcohol for 5 days then cut (1-mm thick) in the coronal plane using an
ISOMET^¯ 1000 saw (Buehler, USA) (Figure
4). After cutting, the slices were attached to aluminum supports (stubs)
with silver- and graphite-based conductive glue to improve the flow of electrons. The
stubs were kept dry with silica gel (Synth^¯ Labsynth, Brazil) and
subsequently sputter-coated in gold, in a SCD 050 Sputter Coater (Bal-Tec™, Germany),
for 350 s. The stubs with the samples were then positioned in an Evo-MA10 electron
microscope (Zeiss™, Germany). The images were obtained with 40× magnification and the
trabecular spaces were analyzed qualitatively.