Ca concentration in serum, feces, diet and femur was determined by atomic absorption spectrophotometry [20] . Lanthanum chloride (6500 mg/L in the final solution) was added as interference suppressor. P and Mg concentration in serum, feces, diet and femur was evaluated following habitual methods using an automated analyzer (Abbott Laboratories, Abbott Park, IL, USA). Serum bone alkaline phosphatase (BAP) (IU/L) was measured using a colorimetric method after bone isoenzyme precipitation with wheat germ lectin [20] . Serum type I collagen telopeptide (CTX) (ng/mL) was assessed employing immunoassay (ELISA) (Rat Laps. Osteometer. BioTech, Herlev, Denmark). Total protein and albumin (Alb) in serum were analyzed following habitual methods using an automated analyzer (Abbott Laboratories, Abbott Park, IL, USA).
Automated analyzer
Manufactured by Abbott
Sourced in United States
The Automated Analyzer is a laboratory instrument designed to perform automated analysis of samples. It is capable of conducting various tests and measurements on a wide range of sample types, such as blood, urine, or other bodily fluids. The core function of the Automated Analyzer is to provide accurate and reproducible results through automated processing and analysis of samples.
Automatically generated - may contain errors
4 protocols using automated analyzer
1
Calcium and Bone Metabolism Evaluation
2
Metabolic and Hormonal Profiling
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Waist circumference and hip circumference were measured for calculation of the WHR. The HOMA-IR was calculated using the following equations [41 (link)]: HOMA-IR = FIns (μU/mL) × fasting blood glucose (FBG) (mmol/L)/22.5. Insulin was measured by ELISA. Free fatty acid (FFA) was measured with a commercial kit. TC, high-density lipoprotein cholesterol (HDL-C), LDL-C, and TG were analyzed using an autoanalyzer. Serum sex hormone including LH, follicle- stimulating sormone (FSH), TEST and progestogen (Prog), prolactin (PRL) and estradiol (E2) were measured with electrochemi-luminescence immunoassay (Roche Diagnostics GmbH). Dehydroepiandrosteronesulfate (DHEA-S) and SHBG were performed using an automated analyzer (Abbott Laboratories, Abbott Park, IL). FAI was calculated as (testosterone/SHBG) × 100. Circulating betatrophin concentration was determined with an ELISA (Phoenix Pharmaceuticals Inc. Belmont, CA, USA) by using the manufacturer’s protocol.
3
Comprehensive Blood and Urine Analysis
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The hematocrit and the blood urea nitrogen (BUN), plasma creatinine (Cre), Hb, and plasma glucose levels were all measured by using an automated analyzer (Abbott Point of Care, Chicago, IL, USA). Plasma erythropoietin levels were measured by an enzyme‐linked immunosorbent assay kit (BioLegend, San Diego, CA, USA). Glucose levels in the urine were measured by using an automated analyzer (Hitachi High‐Technology, Tokyo, Japan).
4
Fasting Blood Biomarkers Evaluation
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All the laboratory results were gathered from clinic database. Blood samples were taken after a 12- h fasting period, fasting blood glucose was measured using the hexokinase method. Blood sapmles for complete blood count were collected in Monovette tubes (Sarstedt, Leicester, United Kingdom) containing ethylenediamine tetraacetic acid anticoagulated. Leukocyte counts were calculated by an automated analyzer (Abbott Laboratory, Illinois, USA). Serum level of CRP was measured by using an Ary360 automatic rate turbidimetric system (Beckman Coulter, Inc., Brea, CA, USA). Cardiac troponin I (cTnI) was measured from lithium-heparinised plasma with AQT90 FLEX TnI immunoassay (Radiometer Medical ApS, Denmark). The upper 99th percentile upper reference limit has been determined as ≤0.023 μg/L.
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