Five soil samples from treated and untreated sites were analyzed for three representative enzymes activities (soil urease (S-UE), soil acid phosphatase (S-ACP) and soil β-glucosidase (S-β-GC). Soil enzyme activities were analysed with the S-UE, S-ACP, and S-β-GC assay kit (Solarbio life Sciences, Beijing, China). S-UE was defined as 1 g of soil which produced 1 μg NH3-N (U/g) daily. S-ACP was considered to be 1 g of soil which liberated 1 nmol phenol at 37℃ (U/g) daily. S-β-GC was considered to be 1 g of soil which produced 1 μmol p-nitroohenol (U/g) daily (Dick et al. 2013 (link)). All enzyme assays were conducted in duplicate.
S β gc assay kit
Manufactured by Solarbio
Sourced in China
The S-β-GC assay kit is a laboratory tool used to detect and quantify the presence of S-β-glucoronidase (S-β-GC) in samples. S-β-GC is an enzyme that plays a role in various biological processes. The kit provides the necessary reagents and protocols to perform this specific enzymatic assay.
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Soil Enzyme Activity Assay
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Soil Enzyme Activity Assay
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Five soil samples from treated and untreated sites were analyzed for three representative enzymes activities (Soil Urease (S-UE), Soil acid phosphatase (S-ACP) and Soil β-glucosidase (S-β-GC). Soil enzyme activities were analysed with the S-UE, S-ACP, and S-β-GC assay kit (Solarbio life Sciences, Beijing, China). S-UE was de ned as 1 g of soil which produced 1µg NH 3 -N (U/g) daily. S-ACP was considered to be 1 g of soil which liberated 1nmol phenol at 37℃ (U/g) daily. S-β-GC was considered to be 1 g of soil which produced 1µmol p-nitroohenol (U/g) daily (Dick et al. 2013) (link). All enzyme assays were conducted in duplicate.
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