Most of the images were acquired at room temperature using a Zeiss LSM980 system, equipped with two PMT and one GaAsPand, a ×40 W objective (C-Apochromat ×40/1.1 W Corr M27, Zeiss); same microscope, but a C-Apochromat ×25/1.515 oil immersion objective was used for the TFM experiments. Camera, filter wheels and shutters were controlled by Zeiss’s ZEN Blue v.3.0. Images in Extended Data Fig. 3 and Extended Data Fig. 6 were acquired with a Leica Stellaris 5 confocal system by using a ×63/1.4 oil immersion objective with ×1 or ×0.75 optical zoom, respectively. Images in Extended Data Fig. 7b were acquired in a Leica Thunder by using a HC PL APO ×20/0.80 PH2 objective. Camera, filter wheels and shutters were controlled by built-in Leica softwares.
Zen blue v 3
Manufactured by Zeiss
ZEN Blue v.3.0 is a software solution for microscope image acquisition, processing, and analysis. It provides a user-interface for controlling Zeiss microscopes and managing image data.
Automatically generated - may contain errors
2 protocols using zen blue v 3
1
Imaging and Microscope Techniques for Cell Biology
2
Microscopy Techniques for Cellular Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Time-lapse imaging. Images for dispersion assays were acquired every 5 min at 18 °C using an upright microscope Zeiss Imager Z2/Apotome.2 equipped with a motorized stage and a camera (Hamamatsu Orca flash 4.0 v2). A 10×W objective (N-Achroplan 10x/0.3 M27 (FWD=2.6mm), ZEIZZ) was used.
In situ hybridization imaging. All images were captured at room temperature in agarose dishes containing PBS, using a dissecting microscope (MZ FL III, Leica) equipped with a camera (DFL420, Leica) and imaging software (IM50, Leica). Magnification was 3.2×.
Immunofluorescence imaging. All images were acquired at room temperature using a Zeiss LSM980 system, equipped with two PMT and one GaAsPand, a 40×W objective (C-Apochromat 40x/1.1 W Corr M27, ZEIZZ). Camera, filter wheels, and shutters were controlled by Zeiss's ZEN Blue v3.0.
In situ hybridization imaging. All images were captured at room temperature in agarose dishes containing PBS, using a dissecting microscope (MZ FL III, Leica) equipped with a camera (DFL420, Leica) and imaging software (IM50, Leica). Magnification was 3.2×.
Immunofluorescence imaging. All images were acquired at room temperature using a Zeiss LSM980 system, equipped with two PMT and one GaAsPand, a 40×W objective (C-Apochromat 40x/1.1 W Corr M27, ZEIZZ). Camera, filter wheels, and shutters were controlled by Zeiss's ZEN Blue v3.0.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!