Mcp 1 flex set

Manufactured by BD

The MCP-1 Flex Set is a laboratory equipment designed for the measurement of the chemokine monocyte chemoattractant protein-1 (MCP-1) in various sample types. The set includes the necessary components to perform the assay, such as antibodies, standards, and buffers. The core function of this product is to facilitate the quantitative analysis of MCP-1 levels in biological samples.

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MCP-1 (35 citations) Flex Sets (25 citations) Mouse MCP-1/CCL2 Flex Set (2 citations) Mouse MCP‐1 Flex Set (2 citations)

2 protocols using mcp 1 flex set

Comprehensive Lipid and Cytokine Profiling

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Total cholesterol, HDL-cholesterol (HDL-C), and triglyceride concentrations were measured using colorimetric assays (Wako Diagnostics, Richmond, VA). Plasma IL-6, IL-1β, and MCP-1 were determined via flow cytometry with mouse IL-6 Enhanced Sensitivity Flex Set, IL-1β Enhanced Sensitivity Flex Set, and a MCP-1 Flex Set, respectively (BD Biosciences).

Barrett T.J., Schlegel M., Zhou F., Gorenchtein M., Bolstorff J., Moore K.J., Fisher E.A, & Berger J.S. (2019). Platelet regulation of myeloid suppressor of cytokine signaling 3 accelerates atherosclerosis. Science translational medicine, 11(517), eaax0481.

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TNFα-Induced HUVEC Inflammation Assay

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HUVECs were stimulated for 4 h with DMSO or TNFα ± apabetalone (5 or 20 μM). Surface expression of VCAM-1 and SELE was labeled with FITC conjugated anti-CD106 and APC conjugated anti-CD62E (BD Bioscience) and quantified by flow cytometry. Mean fluorescence intensity (MFI) relative to the unstimulated DMSO control were reported. HUVECs were stimulated for overnight with DMSO or TNFα ± apabetalone (5 or 20 μM). Supernatant was collected and MCP-1 secretion was measured by a cytometric bead array (MCP-1 flex set, BD Bioscience), and detected by flow cytometry.

Tsujikawa L.M., Fu L., Das S., Halliday C., Rakai B.D., Stotz S.C., Sarsons C.D., Gilham D., Daze E., Wasiak S., Studer D., Rinker K.D., Sweeney M., Johansson J.O., Wong N.C, & Kulikowski E. (2019). Apabetalone (RVX-208) reduces vascular inflammation in vitro and in CVD patients by a BET-dependent epigenetic mechanism. Clinical Epigenetics, 11, 102.

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