Puromycin-selected lentivirus-transduced primary astrocytes from both KD and control groups were incubated in medium with the BrdU labeling reagent (Thermo Fisher Scientific) for 60 min and were subsequently fixed with 70% ethanol for 20 min at RT. After washing three times with PBS, cells were treated with 1.5M HCl (Sigma-Aldrich) for 30 min and were stained with mouse BrdU antibody (Cell Signaling), Alexa Fluor 488-conjugated goat anti-mouse IgG (H+L) antibody (Invitrogen), and DAPI. Three independent incubations were carried out and results were obtained by counting from 10 random fields for each experiment. Images were captured using a Zeiss Observer Z1 inverted fluorescence microscope at 20x magnification.
1.5m hcl
Manufactured by Merck Group
1.5M HCl is a laboratory solution containing hydrochloric acid at a concentration of 1.5 molar. It is a commonly used reagent for various analytical and experimental applications in chemical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 1.5m hcl
1
Quantifying Astrocyte Proliferation via BrdU
2
Quantifying Astrocyte Proliferation via BrdU
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