P3hb p 3hb co 3hv

1 H (500 MHz) NMR spectra was recorded on a JNM-ECA500 NMR spectrometer (JEOL, Japan) at 20 °C. Samples of produced PHA and standard P3HB/ P(3HB-co-3HV) (Aldrich) were prepared by dissolving in deuterated chloroform (CDCl3), and then were filtrated with cotton. The signals of tetramethylsilane (TMS) and CDCl3 were used as the standards for chemical shift of 1 H spectra.

Extraction and estimation of PHA was performed following the procedures reported (Law and lepecky, 1960 ; Venkata Mohan and Venkateswar Reddy, 2013; Venkateswar Reddy et al. 2016 ). 1 H (500 MHz) NMR spectra was recorded on a JNM-ECA500 NMR spectrometer (JEOL, Japan) at 20 °C to know the structure of polymer. Samples of produced PHA and standard P3HB/ P(3HB-co-3HV) (Aldrich) were prepared by dissolving in deuterated chloroform (CDCl3), and then were filtrated with cotton. The signals of tetramethylsilane (TMS) and CDCl3 were used as the standards for chemical shift of 1 H spectra.
Number and weight average molecular mass (Mn and Mw) of standard and sample P3HB/ P(3HB-co-3HV) were measured using GPC 900-1 (JASCO, Japan) equipped with two Shodex K-806L columns and an RI detector. Chloroform was used as an eluent at 40 ºC and polystyrene standards (Mn = 1,680-3,065,000) were employed for calibration.