RPMI-1640 media, DMEM media and other necessary chemicals like antibiotics, amino acids, etc. were purchased from HIMEDIA (Mumbai, India). Fetal bovine serum (FBS) was purchased from HyClone (Thermo Scientific Hy-Clone, Logan, Utah, USA). Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was purchased from Sisco Research Laboratory (Mumbai, India). Cisplatin, ribonuclease, Fluorescein isothiocyanate (FITC) conjugated Annexin V, apoptosis detection kit, Z-vad FMK, RNaseA, Bradford reagent, luminol and coumaric acid were purchased from Sigma (Missouri, USA). Antibodies were purchased from abcam (Cambridge, UK) and Cell Signaling Technology (Danvers, MA, USA). Other essential chemicals used in this study were of the analytical grade.
Methylthiazolyldiphenyl tetrazolium bromide mtt
Manufactured by Sisco Research Laboratories
Sourced in India
Methylthiazolyldiphenyl-tetrazolium bromide (MTT) is a colorimetric assay used to measure cell metabolic activity. It is a yellow tetrazolium salt that can be reduced to purple formazan crystals by metabolically active cells, indicating cellular viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Apoptosis detection kit, by Merck Group (2 mentions)
Amino acids, by HiMedia (2 mentions)
Rpmi 1640 media, by HiMedia (2 mentions)
Coumaric acid, by Merck Group (2 mentions)
Fluorescein isothiocyanate fitc conjugated annexin 5, by Merck Group (2 mentions)
Rnase a, by Merck Group (2 mentions)
Luminol, by Merck Group (2 mentions)
Bradford reagent, by Merck Group (2 mentions)
Z vad fmk, by Merck Group (1 mentions)
Cisplatin, by Merck Group (1 mentions)
Dmem media, by HiMedia (1 mentions)
Anti lamin b1, by Cell Signaling Technology (1 mentions)
Ly294002, by Merck Group (1 mentions)
2 protocols using methylthiazolyldiphenyl tetrazolium bromide mtt
1
Cell Viability and Apoptosis Assay
2
Oxidative Stress-Induced Apoptosis Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RPMI-1640 media and other necessary chemicals like antibiotics, amino acids etc were purchased from HIMEDIA (Mumbai, India) and fetal bovine serum (FBS) from HyClone (Thermo Scientific Hy-Clone, Logan, Utah), respectively. tBHP was purchased from Sigma-Aldrich and Methylthiazolyldiphenyl-tetrazolium bromide (MTT) were purchased from Sisco Research Laboratory (Mumbai, India). Fluorescein isothiocyanate (FITC) conjugated Annexin V, Apoptosis detection kit, RNaseA, Bradford reagent, Luminol, Coumaric acid and LY294002 (a PI3K inhibitor) were also purchased from Sigma (Missouri, USA). Antibodies such as anti Caspase-3 (ab47131), anti Caspase-8 (ab25901), anti Bid (ab77815), anti Bcl2 (ab7973), anti cytochrome c (ab76237), anti Nrf2 (ab31163), anti HO1 (ab13243), anti SOD2 (ab13533), Phospho JNK (ab4821), anti Bax (ab32503), anti PI3k (ab74136), anti Akt (ab17785), Phospho Akt (ab23509), HRP (ab97051) were purchased from Abcam (Cambridge, UK). Phospho BAD (#9291), anti mTOR (#2983), anti GSK 3β (5338), anti Cyclin D1 (#2978), anti PARP (#9532), anti Caspase-9 (#9508), anti Apaf-1 (#8723), anti Bax (#2772), Anti NFκB (#3034), anti β-Actin (#4970), anti Lamin B1 (#12586) was purchased from Cell Signaling Technology (Danvers, MA 01923). Other essential chemicals used in this study were of the analytical grade.
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