K 1000

Manufactured by Sysmex

Sourced in Japan, United States, Germany

The Sysmex K-1000 is a compact, fully automated hematology analyzer designed to perform complete blood count (CBC) analysis. It measures and provides quantitative results for various red blood cell, white blood cell, and platelet parameters.

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27 protocols using k 1000

Hematological and Biochemical Analyses in Fasted Rats

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After 45 days, 12 h-fasted animals were anesthetized with ether and the blood collected with and without anticoagulant (ethylene diamine tetra acetate) by retro-orbital puncture, using capillary tubes for hematological and biochemical studies, respectively (10 (link)). The following hematological parameters were determined with the Sysmex K-1000 fully automated hematology analyzer: Erythrocyte (RBC), total and differential leukocyte (WBC), neutrophil, lymphocyte, eosinophil, basophil, hematocrit (Hct), hemoglobin (Hb), platelet count, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean platelet volume (MPV), platelet distribution width (PDW), and red distribution width (RDW).
Blood samples for biochemical analyses were centrifuged at 3,000 × g for 5 min and plasma was collected and analyzed for glucose, creatinine, albumin, cholesterol, triglycerides, aspartate amino-transferase (AST), alanine aminotransferase (ALT), phosphorus, potassium, sodium, calcium, urea and total protein using a COBAS Mira S chemistry analyzer (Roche Diagnostic Systems, West Sussex, England).

Modarresi M., Hosseinzadeh L., Nematy N., Siavash-Haghighi Z.M, & Ghanbari K. (2014). Acute and subchronic toxicological evaluation of Stachys lavandulifolia aqueous extract in Wistar rats. Research in Pharmaceutical Sciences, 9(3), 165-172.

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Intracerebroventricular Venom Effects on Hematology

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The crude venom at a concentration of 6.25 mg/kg body weight was administered through intracerebroventricular injection into male Kunming mice, with each weighing 18−20 g. After 1 h and at 7 days, blood samples were collected. The serum was used for enzyme and electrolytical analyses. Potassium oxalate-sodium fluoride was used as an anticoagulant for blood sugar analysis. Various hematological parameters, including total leukocyte count, total erythrocyte count, hemoglobin, hematocrit value, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and platelet count were determined using an automated cell counter (K-1000, Sysmex, Kobe, Japan). Serum glutamic-oxaloacetic transaminase, lactate dehydrogenase, and alkaline phosphatase were assayed using an autoanalyzer (Erba Smart Lab, Daman, India) by using diagnostic kits (Biocon, VoehlMarienhagen, Germany; and Raichem, San Diego, CA, USA). Blood parameters were determined using the methodologies described in the manufacturers’ respective manuals for the analytical instruments.

Zhang G., Jia H., Luo L., Zhang Y., Cen X., Yao G., Zhang H., He M, & Liu W. (2022). Molecular and Functional Characterization of a Novel Kunitz-Type Toxin-like Peptide in the Giant Triton Snail Charonia tritonis. Marine Drugs, 20(11), 686.

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Comprehensive Hematological and Clinical Chemistry Analysis

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Heparinized blood (2.5 mL) was subjected to hematological analysis using an automatic analyzer K-1000 (Sysmex; Kobe, Japan). The following parameters were determined: red blood cell count (RBC), white blood cell count (WBC), hemoglobin concentration (HGB), the hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet count (PLT), and the percentage of differentiated white blood cells including neutrophil (NEUT), lymphocyte (LYMPH), monocyte (MONO), eosinophil (EO), and basophil (BASO).
For clinical chemistry determination, serum was prepared from nonheparinized blood (5 mL). Glucose (GLU), blood urea nitrogen (BUN), creatinine (CRE), total protein (TP), albumin (ALB), total bilirubin (T-BIL), direct bilirubin (D-BIL), serum glutamic-oxaloacetic transaminase (SGOT), serum glutamine-pyruvic transaminase (SGPT), and alkaline phosphatase (ALP) were measured. These parameters were determined by the COBAS INTEGRA system (Roche Diagnostic System, Indianapolis, IN).

Koontongkaew S., Poachanukoon O., Sireeratawong S., Dechatiwongse Na Ayudhya T., Khonsung P., Jaijoy K., Soawakontha R, & Chanchai M. (2014). Safety Evaluation of Zingiber cassumunar Roxb. Rhizome Extract: Acute and Chronic Toxicity Studies in Rats. International Scholarly Research Notices, 2014, 632608.

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Comprehensive Blood Panel Analysis

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The following hematological parameters were determined in EDTA whole blood by using an automatic blood cell counter (Sysmex K1000, Germany): RBC, white blood cells (WBC), and platelet and reticulocyte count, hematocrit (HCT) and hemoglobin (HGB) concentration, and hematimetric indices (mean cell volume [MCV], mean cell hemoglobin [MCH], and mean cell hemoglobin concentration [MCHC]). The following biochemical parameters were assessed in serum by using automatic validated methods and equipments (Hitachi 717 analyzer, Roche Diagnostics Inc., MA, USA): glucose, urea, creatinine, uric acid, aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglycerides (TGs), and total cholesterol (Total-Chol).

Madureira A.R., Nunes S., Campos D.A., Fernandes J.C., Marques C., Zuzarte M., Gullón B., Rodríguez-Alcalá L.M., Calhau C., Sarmento B., Gomes A.M., Pintado M.M, & Reis F. (2016). Safety profile of solid lipid nanoparticles loaded with rosmarinic acid for oral use: in vitro and animal approaches. International Journal of Nanomedicine, 11, 3621-3640.

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Evaluating the Impact of DBT on Murine Blood and Splenocytes

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Murine blood and spleens were collected to evaluate the influence of DBT to peripheral blood cells and splenocyte differentiation. The counts of peripheral blood cells were calculated by using a Sysmex K-1000 automated hematology analyzer (Sysmex American, Lincolnshire, IL, USA). The differentiation of splenocytes was evaluated by flow cytometry. The spleen was treated with 2 ml RBC lysis buffer to isolate splenocytes. The BD FACSCantoTM II system (BD, San Jose, CA, USA) was used for cytometry. Murine anti-CD3ε (BD, San Jose, CA, USA), anti-CD4 (BD, San Jose, CA, USA), anti-CD8a (BD, San Jose, CA, USA), anti-CD19 (BD, San Jose, CA, USA), and anti-CD56 (Bioss, Woburn, MA, USA) antibodies were used to label total T, Th, cytotoxic T (Tc), B, and natural killer (NK) cells, respectively. The antibodies were stained by FITC (fluorescein isothiocyanate) and PE (phycoerythrin).

Wu T.H., Yeh K.Y., Wang C.H., Wang H., Li T.L., Chan Y.L, & Wu C.J. (2019). The Combination of Astragalus membranaceus and Angelica sinensis Inhibits Lung Cancer and Cachexia through Its Immunomodulatory Function. Journal of Oncology, 2019, 9206951.

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Automated Platelet Enumeration Protocol

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The number of platelets was determined using an automated electronic particle counter (Sysmex, K-1000, and Kobe, Japan). For this purpose, 0.1 ml of PC was added to 0.4 ml of phosphate buffer saline (PBS).

Mirshafiee H., Sharifi Z., Hosseini S.M., Yari F., Nikbakht H, & Latifi H. (2015). The Effects of Ultraviolet Light and Riboflavin on Inactivation of Viruses and the Quality of Platelet Concentrates at Laboratory Scale. Avicenna Journal of Medical Biotechnology, 7(2), 57-63.

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Automated Blood Cell Analysis

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Two ml of blood was withdrawn by venipuncture using a sterile plastic syringe. This sample of blood was then delivered into a sterile plastic tube containing EDTA (disodium ethylenediaminetetraacetate) as an anticoagulant, then introduced into automatized counter (Sysmex K 1000) which directly gives Hb concentration, WBCs, RBCs, and platelets counts.

Kasemy Z.A., Kamel G.M., Abdel-Rasoul G.M, & Ismail A.A. (2019). Environmental and Health Effects of Benzene Exposure among Egyptian Taxi Drivers. Journal of Environmental and Public Health, 2019, 7078024.

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Comprehensive Metabolic Profile Analysis

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Fasting blood samples of approximately 5 ml for measuring complete blood count (Sysmex K-1000, Bohemia, NY, USA) and other factors were immediately centrifuged at 3000 g for 10 min. Serum levels of blood urea nitrogen (BUN), creatinine (Cre), fasting glucose, total cholesterol (TCH), triglyceride (TG), high-density lipoprotein cholesterol (HDL-cholesterol), and low-density lipoprotein cholesterol (LDL-cholesterol) were measured using an autoanalyzer (COBAS Integra 800, Roche Diagnostics, Basel, Switzerland). Blood samples were assayed for NT-proBNP by electrochemiluminescence immunoassay on the Elecsys 2010 Immunoanalyzer (Roche Diagnostics, Indianapolis, IN, USA).

Wang J.H., Lee C.J., Hsieh J.C., Chen Y.C, & Hsu B.G. (2014). N-terminal pro-B-type natriuretic peptide level inversely associates with metabolic syndrome in elderly persons. Diabetology & Metabolic Syndrome, 6, 15.

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Exercise-Induced Changes in Blood Cell Counts and CK-MM

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The blood samples were collected before exercise, 12 h and 24 h after exercise. These samples were placed into heparinized tubes and measured immediately for blood cell counts (Sysmex K-1000, NY, United States). The samples were then centrifuged at 3,000 × g for 10 min. After centrifugation, supernatant was collected and the level of CK-MM was measured within 1 hour by using an automatic biochemical analyzer (COBAS INTEGRA 800, Roche Diagnostics, Basel, Switzerland).

Lu C.C., Ke C.Y., Wu W.T, & Lee R.P. (2023). L-Glutamine is better for treatment than prevention in exhaustive exercise. Frontiers in Physiology, 14, 1172342.

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Comprehensive Blood Profiling Protocol

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Total and differential leukocyte counts (neutrophils, lymphocytes, and a group of mixed cell types including monocytes, eosinophils and basophils) were counted in heparinized blood samples using a SYSMEX K1000 automatic counter (Sysmex Europe, Norderstedt, Germany). The analyses were performed consecutively at the time of the screening examination, at the central laboratory of Malmö University Hospital.
HbA1c and whole blood glucose was measured according to standard procedures at the Department of Clinical Chemistry. HbA_1c was measured by ion exchange chromatography, with reference values of 3.9–5.3% in non-diabetic individuals. Insulin was measured by a radioimmunoassay in mIU/ L and the HOMA index was calculated as fasting insulin*glucose/22.5 [16 (link)]. CRP was analyzed using a high-sensitive assay, Tina-quant^® CRP latex assay (Roche Diagnostics, Basel, Switzerland).

Borné Y., Smith J.G., Nilsson P.M., Melander O., Hedblad B, & Engström G. (2016). Total and Differential Leukocyte Counts in Relation to Incidence of Diabetes Mellitus: A Prospective Population-Based Cohort Study. PLoS ONE, 11(2), e0148963.

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