DXR was obtained as doxorubicin hydrochloride (50mg/vial) from Pfizer Pharma. All other chemicals used in this study were of analytical grade. 2- thiobarbituric acid (TBA) and enzyme glutathione reductase (GR) was procured from Sigma-Aldrich Chemical Company (St. Lous, MO, USA). NADPH, CDNB, glutathione oxidized, glutathione reduced, hydroxylamine HCl, Triton X-100, EDTA, NBT and DTNB were purchased from SRL Chemicals (India). serum glutamic pyruvic transaminase (SGPT), serum glutamic oxaloacetic transaminase (SGOT) and Bilirubin assay kits were procured from Reckon Diagnostics private Ltd. India.
Nadph
Manufactured by Sisco Research Laboratories
Sourced in India
NADPH is a cofactor essential for various enzymatic reactions in cells. It is the reduced form of nicotinamide adenine dinucleotide phosphate (NADP+) and plays a crucial role in cellular metabolism, antioxidant defense, and biosynthetic pathways.
Automatically generated - may contain errors
Lab products found in correlation
Tris hcl, by Merck Group (3 mentions)
Glutathione oxidized, by Sisco Research Laboratories (2 mentions)
Reduced glutathione, by Sisco Research Laboratories (2 mentions)
Ethylene diamine tetraacetic acid (edta), by Sisco Research Laboratories (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
N n dimethylformamide (dmf), by Merck Group (2 mentions)
2 thiobarbituric acid tba, by Merck Group (1 mentions)
Doxorubicin hydrochloride, by Pfizer (1 mentions)
Xanthine, by Merck Group (1 mentions)
Xanthine oxidase, by Merck Group (1 mentions)
Lps l4391, by Merck Group (1 mentions)
β mercaptoethanol, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Ammonium persulfate, by Merck Group (1 mentions)
Thiobarbituric acid, by Merck Group (1 mentions)
Cytochrome c, by Merck Group (1 mentions)
Acrylamide, by Merck Group (1 mentions)
Glycine, by Merck Group (1 mentions)
Sodium nitrate, by Sisco Research Laboratories (1 mentions)
Adenosine diphosphate (adp), by Merck Group (1 mentions)
4 protocols using nadph
1
Doxorubicin-Induced Liver Injury Assay
2
Characterization of Drug Interactions
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Imipramine (IMI) was obtained from S. D. Fine-Chem Ltd, Mumbai. Metoprolol succinate (MET) was obtained as a gift sample from Ipca Laboratories Ltd, Mumbai. Clomipramine hydrochloride was obtained as a gift sample from Cipla Pvt. Ltd, Mumbai. Pindolol and bovine serum albumin were gift samples obtained from Piramal Life Science Pvt. Ltd., Mumbai. NADPH was obtained from SRL Chemicals Ltd., Mumbai. Tris-HCl was obtained from Sigma Chemicals Co., USA. Sucrose AR, p-nitrophenol, glycerol, ethylenediamine tetraacetic acid AR, dipotassium hydrogen phosphate AR, Triton X-100, sodium dithionite purified, ethanol AR, coomassie brilliant blue G-250, phosphoric acid (85% w/v), conc. HCl, acetone, dioxane, n-propanol and PEG 400 were obtained from S. D. Fine-Chem. Ltd. Mumbai. Calcium chloride AR was obtained from Thomas Baker. Carbon monoxide gas was obtained from Alchemi Gases and Chemicals Pvt. Ltd. Mumbai. ACN, MeOH (HPLC grade), diethyl ether LR was obtained from Qualigens Ltd., Mumbai, DMF and DMSO were obtained from Merck India Ltd. All other chemicals were of analytical grade.
3
Purification and Characterization of Enzyme
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p-Nitrocatechol was obtained from Lancaster Chemicals, Mumbai. Salicylamide and NADPH were obtained from SRL Chemicals Ltd., Mumbai. Tris-HCl was obtained from Sigma Chemicals Co., USA. Sucrose AR, p-nitrophenol, glycerol, ethylenediaminetetraacetic acid AR, dipotassium hydrogen phosphate AR, Triton X-100, sodium dithionite purified, ethanol AR, coomassie brilliant blue G-250, phosphoric acid (85% w/v), conc. HCl, acetone, dioxane, n-propanol and PEG 400 were obtained from S. D. Fine-Chem. Ltd. Mumbai. Calcium chloride AR was obtained from Thomas Baker. Carbon monoxide gas was obtained from Alchemi Gases and Chemicals Pvt. Ltd. Mumbai. Bovine serum albumin was obtained as a gift sample from Piramal Life Sciences Ltd. ACN, MeOH (HPLC grade), diethyl ether LR was obtained from Qualigens Ltd., Mumbai, DMF and DMSO were obtained from Merck India Ltd. All other chemicals were of analytical grade.
4
Antioxidant and Anti-inflammatory Assays
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Acrylamide, ammonium per-sulfate, adenosine diphosphate, BSA, beta-mercaptoethanol, CDNB, cytochrome-C, glycine, thiobarbituric acid, Tris-HCl, xanthine, LPS (L4391), and xanthine oxidase were obtained from Sigma Chemicals, St. Louis, MO, USA. Dinitrophenyl hydrazine, EDTA, glutathione oxidized, glutathione reduced, malonaldehyde, NADPH, phosphoric acid, sulphanilamide, sulphosalicylic acid, sodium nitrate, t-butyl hydroperoxide were obtained from SRL Chemicals, Mumbai, India. The ELISA kit for measurement of COX-2 activity was purchased from Cayman Chemicals, Ann Arbor, MI, USA. Zerumbone (99.9% pure) was extracted from ginger as per the earlier procedure [15] .
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