Polycarbonate membrane
Polycarbonate membranes are thin, flat, and porous membranes made from polycarbonate material. They are primarily used as filtration and separation media in various laboratory applications.
Lab products found in correlation
29 protocols using polycarbonate membrane
Preparation of Lipid Vesicles for Biophysical Studies
Preparation of Cationic Liposomes for Delivery
Liposome Preparation via Thin Film Hydration
Preparation of DOPS Liposomes
Preparation of DPPC Liposomes
Preparation of DMPC Liposomal Vesicles
In-Vitro Antigen Release Study
Synthesis of Gold-Loaded Liposomes
Liposome-Mediated Protein Encapsulation and Delivery
The dequenching of ANTS fluorescence was measured in an ISS K2 multiphase frequency and modulation fluorometer (ISS, Champaign, IL, USA) with excitation at 380 nm and emission at 520 nm. 100 μL of liposome and 100 μg of protein were mixed with the assay buffer (150mM NaCl, 20mM Tris, pH7.4) to a final volume of 1350 μL. After 30 s of incubation, 150 μL of 1 M NaAc solution (pH 4.0) was added into the mixture to activate acidic pH-dependent membrane insertion, and the fluorescence intensity was recorded continuously for the following 180 s. The mixture was continuously stirred throughout the assay. Prior to the assay, EsxA-ST was incubated with SC-GFP (molar ratio 1:0.5) for 2 hours at RT to allow formation of the covalent bond between EsxA-ST and SC-GFP.
Reconstitution of Ciliary Membrane Lipid Composition in Liposomes
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!