Sodium thiopental

Example 3

Guinea pigs (Hartley, body weight: 420 g to 650 g, male, obtained from Japan SLC, Inc.) were each anesthetized by intraperitoneally administering thereto sodium thiopental (product of Mitsubishi Tanabe Pharma Corporation) at 50 mg/kg. Next, a tracheal cannula was inserted into each of the guinea pigs. While their body temperature was being maintained at 37° C. with a warming pad, the guinea pigs were allowed to inhale 100% by volume oxygen and 1% by volume vaporized halothane (product of Takeda Pharmaceutical Company Limited.) using an artificial respirator for experimental animals (SN-480-7; product of shimano Co., Ltd.). Here, the artificial respirator for experimental animals was set to a tidal volume of 10 mL/kg and a respiration rate of 60 strokes/min. Next, a cannula for drug administration was inserted to their left femoral vein. In addition, a heparinized catheter for monitoring the blood pressure was inserted to the aorta through the left femoral vein. Then, ECG standard limb II leads was continuously recorded.

Example 1

Extremely small-size miniature pigs (trade name: Microminipig, body weight: about 10 kg, male, obtained from Fuji Micra Inc.) were each anesthetized by allowing them to inhale 100% by volume oxygen and 4% by volume vaporized halothane (product of Takeda Pharmaceutical Company Limited.). After insertion of a tracheal cannula, an artificial respirator was used to ventilate it with 100% by volume oxygen and 1% by volume vaporized halothane. Here, the artificial respirator for experimental animals was set to a tidal volume of 10 mL/kg and a respiration rate of 20 strokes/min. Next, a cannula for drug administration was inserted to their left femoral vein. Then, ECG standard limb II leads was continuously recorded.

Dogs (body weight: 10 kg, male, obtained from Nosan Co., Ltd.) were each anesthetized by intravenously administering thereto sodium thiopental (product of Mitsubishi Tanabe Pharma Corporation) at 30 mg/kg. After insertion of a tracheal cannula, an artificial respirator was used to ventilate it with 100% by volume oxygen and 1% by volume vaporized halothane. Here, the artificial respirator for experimental animals was set to a tidal volume of 20 mL/kg and a respiration rate of 15 strokes/min. Next, a cannula for drug administration was inserted to their left femoral vein. Then, ECG standard limb II leads was continuously recorded.

Example 2

Monkeys (Macaca fascicularis, body weight: about 3 kg, male, produced in China, purchased from a domestic breeder) were each anesthetized by intravenously administering thereto sodium thiopental (product of Mitsubishi Tanabe Pharma Corporation) at 25 mg/kg. After insertion of a tracheal cannula, an artificial respirator was used to ventilate it with 100% by volume oxygen and 1% by volume vaporized halothane. Here, the artificial respirator for experimental animals was set to a tidal volume of 20 mL/kg and a respiration rate of 15 strokes/min. Next, a cannula for drug administration was inserted to their left femoral vein. Then, ECG standard limb II leads was continuously recorded.