MOLM-13 (ATCC, Manassas, VA) cells were maintained in RPMI-1640 (Fisher Scientific, Waltham, MA) with 10% fetal bovine serum (Fisher Scientific, Waltham, MA). DS-3032b (milademetan) or DS-5272 (provided by Daiichi-Sankyo, Chuo, Japan) was dissolved in DMSO and added to cells one day before AZA (Sigma-Aldrich, St. Louis, MO) treatment, which lasted for three days. The coefficient of drug interaction (CDI) is calculated as follows: CDI = AB/(A×B). AB is the ratio of the combination groups to control group; A or B is the ratio of the single agent group to control group. Thus, CDI value <1 indicates that the drugs are synergistic [20 (link)].
Ds 3032b
Manufactured by Daiichi Sankyo
The DS-3032b is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 3,200 rpm and a maximum relative centrifugal force (RCF) of 1,760 x g. The centrifuge is equipped with a fixed-angle rotor that can accommodate multiple sample tubes or microplates. The device is suitable for a variety of sample preparation and separation tasks in research and diagnostic laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Ds 5272, by Daiichi Sankyo (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Molm 13, by American Type Culture Collection (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Ovtoko, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Caki 1, by American Type Culture Collection (1 mentions)
Ovise, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
Caki 2, by American Type Culture Collection (1 mentions)
Jhoc 7, by RIKEN Cell Bank (1 mentions)
Jhoc 9, by RIKEN Cell Bank (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Everolimus, by LC Laboratories (1 mentions)
Nutlin 3a, by Cayman Chemical (1 mentions)
3 protocols using ds 3032b
1
Synergistic Evaluation of DS-3032b and Azacitidine in MOLM-13 Cells
2
Comparative Analysis of CCC Cell Lines
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We used 6 human CCOC cell lines (OVISE, OVTOKO, JHOC‐7, JHOC‐9, ES‐2, and SKOV‐3), 2 CCRC cell lines (Caki‐1 and Caki‐2), and 1 mouse ovarian carcinoma cell line (ID8). The OVISE and OVTOKO cell lines were purchased from the Japanese Collection of Research Bioresources Cell Bank. JHOC‐7 and JHOC‐9 cell lines were purchased from the RIKEN Cell Bank. ES‐2, SKOV3, Caki‐1, and Caki‐2 cell lines were purchased from ATCC. ID8 cells were kindly gifted by Dr Kathy Roby of the Department of Anatomy and Cell Biology at the University of Kansas Medical Center. All cell lines were cultured at 37°C in a humidified incubator with 5% CO2. All 9 cell lines were classified histologically as CCCs and authenticated by short tandem repeat analysis. We used the International Cell Line Authentication Committee database to confirm that these cell lines were not cross‐contaminated or misidentified. The MDM2 inhibitors DS‐3032b and DS‐5272 were provided by the Daiichi‐Sankyo Company, and the mTOR inhibitor everolimus was purchased from LC Laboratories.
3
Synthesis and Characterization of DS-5272 and DS-3032 Compounds
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